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dc.contributor.authorSmith, Muireann K.*
dc.contributor.authorDraper, Lorraine A.*
dc.contributor.authorHazelhoff, Pieter-Jan*
dc.contributor.authorCotter, Paul D.*
dc.contributor.authorRoss, R Paul*
dc.contributor.authorHill, Colin*
dc.date.accessioned2017-03-08T16:29:52Z
dc.date.available2017-03-08T16:29:52Z
dc.date.issued30/11/2016
dc.identifier.citationSmith Muireann K., Draper Lorraine A., Hazelhoff Pieter-Jan, Cotter Paul D., Ross R. P., Hill Colin. A Bioengineered Nisin Derivative, M21A, in Combination with Food Grade Additives Eradicates Biofilms of Listeria monocytogenes . Frontiers in Microbiology , 2016, 7, 1939. DOI 10.3389/fmicb.2016.01939en_GB
dc.identifier.urihttp://hdl.handle.net/11019/1107
dc.descriptionpeer-revieweden_GB
dc.description.abstractThe burden of foodborne disease has large economic and social consequences worldwide. Despite strict regulations, a number of pathogens persist within the food environment, which is greatly contributed to by a build-up of resistance mechanisms and also through the formation of biofilms. Biofilms have been shown to be highly resistant to a number of antimicrobials and can be extremely difficult to remove once they are established. In parallel, the growing concern of consumers regarding the use of chemically derived antimicrobials within food has led to a drive toward more natural products. As a consequence, the use of naturally derived antimicrobials has become of particular interest. In this study we investigated the efficacy of nisin A and its bioengineered derivative M21A in combination with food grade additives to treat biofilms of a representative foodborne disease isolate of Listeria monocytogenes. Investigations revealed the enhanced antimicrobial effects, in liquid culture, of M21A in combination with citric acid or cinnamaldehyde over its wild type nisin A counterpart. Subsequently, an investigation was conducted into the effects of these combinations on an established biofilm of the same strain. Nisin M21A (0.1 μg/ml) alone or in combination with cinnamaldehyde (35 μg/ml) or citric acid (175 μg/ml) performed significantly better than combinations involving nisin A. All combinations of M21A with either citric acid or cinnamaldehyde eradicated the L. monocytogenes biofilm (in relation to a non-biofilm control). We conclude that M21A in combination with available food additives could further enhance the antimicrobial treatment of biofilms within the food industry, simply by substituting nisin A with M21A in current commercial products such as Nisaplin® (Danisco, DuPont).en_GB
dc.language.isoenen_GB
dc.publisherFrontiersen_GB
dc.relation.ispartofseriesFrontiers in Microbiology;vol 7
dc.subjectListeria monocytogenesen_GB
dc.subjectBiofilmen_GB
dc.subjectNisinen_GB
dc.subjectBioengineereden_GB
dc.subjectCitric aciden_GB
dc.subjectcinnamaldehydeen_GB
dc.subjectcombinationsen_GB
dc.titleA Bioengineered Nisin Derivative, M21A, in Combination with Food Grade Additives Eradicates Biofilms of Listeria monocytogenesen_GB
dc.typeArticleen_GB
dc.identifier.doihttp://dx.doi.org/10.3389/fmicb.2016.01939
dc.contributor.sponsorScience Foundation Ireland
dc.contributor.sponsorGrantNumberSFI/12/RC/2273
refterms.dateFOA2018-01-12T08:38:23Z


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