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dc.contributor.authorBurke, D.G.*
dc.contributor.authorFouhy, Fiona*
dc.contributor.authorHarrison, M. J*
dc.contributor.authorRea, Mary*
dc.contributor.authorCotter, Paul D.*
dc.contributor.authorO'Sullivan, Orla*
dc.contributor.authorSTANTON, CATHERINE*
dc.contributor.authorHill, Cian J*
dc.contributor.authorShanahan, Fergus*
dc.contributor.authorPlant, Barry J.*
dc.contributor.authorRoss, R Paul*
dc.date.accessioned2017-03-15T17:14:12Z
dc.date.available2017-03-15T17:14:12Z
dc.date.issued09/03/2017
dc.identifier.citationBurke, D.G. et al, The altered gut microbiota in adults with cystic fibrosis, BMC Microbiology. 2017 Mar 09;17(1):58. DOI: 10.1186/s12866-017-0968-8en_GB
dc.identifier.urihttp://hdl.handle.net/11019/1112
dc.descriptionThe authors and their work were supported by the Science Foundation of Ireland and funded by the Centre for Science, Engineering and Technology (SFI-CSET) grant 02/CE/B124 and by FP7 funded CFMATTERS (Cystic Fibrosis Microbiome-determined Antibiotic Therapy Trial in Exacerbations: Results Stratified, Grant Agreement no. 603038). The APC Microbiome Institute is a research centre funded by Science Foundation Ireland (SFI).
dc.descriptionpeer-revieweden_GB
dc.description.abstractBackground Cystic Fibrosis (CF) is an autosomal recessive disease that affects the function of a number of organs, principally the lungs, but also the gastrointestinal tract. The manifestations of cystic fibrosis transmembrane conductance regulator (CFTR) dysfunction in the gastrointestinal tract, as well as frequent antibiotic exposure, undoubtedly disrupts the gut microbiota. To analyse the effects of CF and its management on the microbiome, we compared the gut microbiota of 43 individuals with CF during a period of stability, to that of 69 non-CF controls using 454-pyrosequencing of the 16S rRNA gene. The impact of clinical parameters, including antibiotic therapy, on the results was also assessed. Results The CF-associated microbiome had reduced microbial diversity, an increase in Firmicutes and a reduction in Bacteroidetes compared to the non-CF controls. While the greatest number of differences in taxonomic abundances of the intestinal microbiota was observed between individuals with CF and the healthy controls, gut microbiota differences were also reported between people with CF when grouped by clinical parameters including % predicted FEV1 (measure of lung dysfunction) and the number of intravenous (IV) antibiotic courses in the previous 12 months. Notably, CF individuals presenting with severe lung dysfunction (% predicted FEV1 ≤ 40%) had significantly (p < 0.05) reduced gut microbiota diversity relative to those presenting with mild or moderate dysfunction. A significant negative correlation (−0.383, Simpson’s Diversity Index) was also observed between the number of IV antibiotic courses and gut microbiota diversity. Conclusions This is one of the largest single-centre studies on gut microbiota in stable adults with CF and demonstrates the significantly altered gut microbiota, including reduced microbial diversity seen in CF patients compared to healthy controls. The data show the impact that CF and it's management have on gut microbiota, presenting the opportunity to develop CF specific probiotics to minimise microbiota alterations.en_GB
dc.description.sponsorshipScience Foundation Irelanden_GB
dc.language.isoenen_GB
dc.publisherBiomed Centralen_GB
dc.relation.ispartofseriesBMC Microbiology;vol 17
dc.subjectCystic Fibrosisen_GB
dc.subjectGut microbiotaen_GB
dc.subject454-pyrosequencingen_GB
dc.subjectMicrobial diversityen_GB
dc.subjectAntibiotic therapyen_GB
dc.titleThe altered gut microbiota in adults with cystic fibrosisen_GB
dc.typeArticleen_GB
dc.date.updated2017-03-09T17:03:06Z
dc.language.rfc3066en
dc.rights.holderThe Author(s).
dc.identifier.doihttp://dx.doi.org/10.1186/s12866-017-0968-8
dc.contributor.sponsorScience Foundation Ireland
refterms.dateFOA2018-01-12T08:37:29Z


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