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dc.contributor.authorHill, Cian J
dc.contributor.authorLynch, Denise B
dc.contributor.authorMurphy, Kiera
dc.contributor.authorUlaszewska, Marynka
dc.contributor.authorJeffery, Ian B
dc.contributor.authorO’Shea, Carol A
dc.contributor.authorWatkins, Claire
dc.contributor.authorDempsey, Eugene
dc.contributor.authorMattivi, Fulvio
dc.contributor.authorTuohy, Kieran
dc.contributor.authorRoss, R. Paul
dc.contributor.authorRyan, C. A
dc.contributor.authorO’ Toole, Paul W
dc.contributor.authorStanton, Catherine
dc.date.accessioned2017-05-24T11:47:36Z
dc.date.available2017-05-24T11:47:36Z
dc.date.issued17/01/2017
dc.identifier.citationhill, Cian J. et al. Evolution of gut microbiota composition from birth to 24 weeks in the INFANTMET Cohort. Microbiome. 2017 Jan 17;5(1):4. DOI: 10.1186/s40168-016-0213-yen_GB
dc.identifier.urihttp://hdl.handle.net/11019/1123
dc.identifier.urihttp://dx.doi.org/10.1186/s40168-016-0213-y
dc.descriptionThis work was supported by the Government of Ireland National Development Plan by way of a Department of Agriculture, Food and Marine FIRM grant to the INFANTMET project (CS), by a grant from the Health Research Board of Ireland (HRA_POR/2012/123) to PWOT, by the APC Microbiome Institute, and by the BIO-IT Platform.
dc.descriptionpeer-revieweden_GB
dc.description.abstractBackground The gut is the most extensively studied niche of the human microbiome. The aim of this study was to characterise the initial gut microbiota development of a cohort of breastfed infants (n = 192) from 1 to 24 weeks of age. Methods V4-V5 region 16S rRNA amplicon Illumina sequencing and, in parallel, bacteriological culture. The metabolomic profile of infant urine at 4 weeks of age was also examined by LC-MS. Results Full-term (FT), spontaneous vaginally delivered (SVD) infants’ microbiota remained stable at both phylum and genus levels during the 24-week period examined. FT Caesarean section (CS) infants displayed an increased faecal abundance of Firmicutes (p < 0.01) and lower abundance of Actinobacteria (p < 0.001) after the first week of life compared to FT-SVD infants. FT-CS infants gradually progressed to harbouring a microbiota closely resembling FT-SVD (which remained stable) by week 8 of life, which was maintained at week 24. The gut microbiota of preterm (PT) infants displayed a significantly greater abundance of Proteobacteria compared to FT infants (p < 0.001) at week 1. Metabolomic analysis of urine at week 4 indicated PT-CS infants have a functionally different metabolite profile than FT (both CS and SVD) infants. Co-inertia analysis showed co-variation between the urine metabolome and the faecal microbiota of the infants. Tryptophan and tyrosine metabolic pathways, as well as fatty acid and bile acid metabolism, were found to be affected by delivery mode and gestational age. Conclusions These findings confirm that mode of delivery and gestational age both have significant effects on early neonatal microbiota composition. There is also a significant difference between the metabolite profile of FT and PT infants. Prolonged breastfeeding was shown to have a significant effect on the microbiota composition of FT-CS infants at 24 weeks of age, but interestingly not on that of FT-SVD infants. Twins had more similar microbiota to one another than between two random infants, reflecting the influence of similarities in both host genetics and the environment on the microbiota.en_GB
dc.description.sponsorshipHealth Research Board of Ireland
dc.description.sponsorshipAPC Microbiome Institute
dc.description.sponsorshipBIO-IT Platform
dc.description.sponsorshipDepartment of Agriculture, Food and the Marineen_GB
dc.language.isoenen_GB
dc.publisherBiomed Centralen_GB
dc.relation.ispartofseriesMicrobiome;
dc.subjectGut microbiotaen_GB
dc.subjectNeonatalen_GB
dc.subjectINFANTMETen_GB
dc.subjectBreastfeedingen_GB
dc.titleEvolution of gut microbiota composition from birth to 24 weeks in the INFANTMET Cohorten_GB
dc.typeArticleen_GB
dc.date.updated2017-05-21T03:33:07Z
dc.language.rfc3066en
dc.rights.holderThe Author(s).
refterms.dateFOA2018-01-12T08:54:17Z


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