Show simple item record

dc.contributor.authorDeakin, Gregory*
dc.contributor.authorDobbs, Edward*
dc.contributor.authorBennett, Julie M.*
dc.contributor.authorJones, Ian M.*
dc.contributor.authorGrogan, Helen*
dc.contributor.authorBurton, Kerry S.*
dc.date.accessioned2017-07-24T14:10:26Z
dc.date.available2017-07-24T14:10:26Z
dc.date.issued26/05/2017
dc.identifier.citationDeakin G, Dobbs E, Bennett JM, Jones IM, Grogan HM, Burton KS. Multiple viral infections in Agaricus bisporus - Characterisation of 18 unique RNA viruses and 8 ORFans identified by deep sequencing. Scientific Reports 2017;7; doi 10.1038/s41598-017-01592-9.en_GB
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11019/1212
dc.descriptionpeer-revieweden_GB
dc.description.abstractThirty unique non-host RNAs were sequenced in the cultivated fungus, Agaricus bisporus, comprising 18 viruses each encoding an RdRp domain with an additional 8 ORFans (non-host RNAs with no similarity to known sequences). Two viruses were multipartite with component RNAs showing correlative abundances and common 3′ motifs. The viruses, all positive sense single-stranded, were classified into diverse orders/families. Multiple infections of Agaricus may represent a diverse, dynamic and interactive viral ecosystem with sequence variability ranging over 2 orders of magnitude and evidence of recombination, horizontal gene transfer and variable fragment numbers. Large numbers of viral RNAs were detected in multiple Agaricus samples; up to 24 in samples symptomatic for disease and 8–17 in asymptomatic samples, suggesting adaptive strategies for co-existence. The viral composition of growing cultures was dynamic, with evidence of gains and losses depending on the environment and included new hypothetical viruses when compared with the current transcriptome and EST databases. As the non-cellular transmission of mycoviruses is rare, the founding infections may be ancient, preserved in wild Agaricus populations, which act as reservoirs for subsequent cell-to-cell infection when host populations are expanded massively through fungiculture.en_GB
dc.description.sponsorshipThis research has received funding from the Teagasc Walsh Fellowship Programme, (Project 201043) and the European Union Seventh Framework Programme (FP7-SME-2011) under grant agreement No. 286836 (MushTV).en_GB
dc.language.isoenen_GB
dc.publisherNature Publishing Groupen_GB
dc.relation.ispartofseriesScientific Reports;vol 7
dc.subjectRNA sequencingen_GB
dc.subjectViral pathogenesisen_GB
dc.subjectAgaricus bisporusen_GB
dc.titleMultiple viral infections in Agaricus bisporus - Characterisation of 18 unique RNA viruses and 8 ORFans identified by deep sequencingen_GB
dc.typeArticleen_GB
dc.identifier.doihttp://dx.doi.org/10.1038/s41598-017-01592-9
dc.contributor.sponsorTeagasc Walsh Fellowship Programme
dc.contributor.sponsorEuropean Commission
dc.contributor.sponsorGrantNumber201043
dc.contributor.sponsorGrantNumber286836
refterms.dateFOA2018-01-12T08:54:53Z


Files in this item

Thumbnail
Name:
MultipleVirusInfectionsAgaricu ...
Size:
2.402Mb
Format:
PDF
Description:
Main article
Thumbnail
Name:
SupplementaryInfoMultipleVirus ...
Size:
758.9Kb
Format:
PDF
Description:
Supplementary information

This item appears in the following Collection(s)

Show simple item record