O'Connor, Paula M.
Rea, Mary C.
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CitationGough, R., Gómez-Sala, B., O’Connor, P. M., Rea, M. C., Miao, S., Hill, C., & Brodkorb, A. (2017). A Simple Method for the Purification of Nisin. Probiotics and Antimicrobial Proteins, 9(3), 363-369. doi: 10.1007/s12602-017-9287-5
AbstractNisin, an antimicrobial peptide showing activity against a broad range of Gram-positive bacteria, is widely used as a food preservative and has potential as a therapeutic for a range of infectious diseases. Here, we present a simple purification method, based on a salting-out approach, which can produce a powder containing ∼33% nisin, from a nisin-producing culture in a whey permeate-based medium. This process removes over 99% of the lactic acid, NaCl, lactose and non-nisin proteins from the cell-free culture supernatant. The approach can also enrich a commonly used commercial nisin preparation over 30-fold to a purity of ∼58%. These are higher purities than comparable published methods. The simplicity of this approach facilitates its use in research and also its scale-up.