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dc.contributor.authorKeogh, Kate*
dc.contributor.authorWaters, Sinead M.*
dc.contributor.authorCormican, Paul*
dc.contributor.authorKelly, Alan K*
dc.contributor.authorO'Shea, Emma*
dc.contributor.authorKenny, David A.*
dc.date.accessioned2017-08-30T13:57:34Z
dc.date.available2017-08-30T13:57:34Z
dc.date.issued2017-05-17
dc.identifier.citationKeogh K, Waters SM, Cormican P, Kelly AK, O’Shea E, et al. (2017) Effect of dietary restriction and subsequent re-alimentation on the transcriptional profile of bovine ruminal epithelium. PLOS ONE 12(5): e0177852. https://doi.org/10.1371/journal.pone.0177852en_GB
dc.identifier.urihttp://hdl.handle.net/11019/1510
dc.descriptionpeer-revieweden_GB
dc.description.abstractCompensatory growth (CG) is utilised worldwide in beef production systems as a management approach to reduce feed costs. However the underlying biology regulating the expression of CG remains to be fully elucidated. The objective of this study was to examine the effect of dietary restriction and subsequent re-alimentation induced CG on the global gene expression profile of ruminal epithelial papillae. Holstein Friesian bulls (n = 60) were assigned to one of two groups: restricted feed allowance (RES; n = 30) for 125 days (Period 1) followed by ad libitum access to feed for 55 days (Period 2) or (ii) ad libitum access to feed throughout (ADLIB; n = 30). At the end of each period, 15 animals from each treatment were slaughtered and rumen papillae harvested. mRNA was isolated from all papillae samples collected. cDNA libraries were then prepared and sequenced. Resultant reads were subsequently analysed bioinformatically and differentially expressed genes (DEGs) are defined as having a Benjamini-Hochberg P value of <0.05. During re-alimentation in Period 2, RES animals displayed CG, growing at 1.8 times the rate of their ADLIB contemporary animals in Period 2 (P < 0.001). At the end of Period 1, 64 DEGs were identified between RES and ADLIB, with only one DEG identified at the end of Period 2. When analysed within RES treatment (RES, Period 2 v Period 1), 411 DEGs were evident. Genes identified as differentially expressed in response to both dietary restriction and subsequent CG included those involved in processes such as cellular interactions and transport, protein folding and gene expression, as well as immune response. This study provides an insight into the molecular mechanisms underlying the expression of CG in rumen papillae of cattle; however the results suggest that the role of the ruminal epithelium in supporting overall animal CG may have declined by day 55 of re-alimentation.en_GB
dc.description.sponsorshipSMW received financial assistance from Science Foundation Ireland (SFI) contract no 09/ RFP/GEN2447.
dc.language.isoenen_GB
dc.publisherPLOSen_GB
dc.relation.ispartofseriesPLoS ONE;vol 12
dc.subjectCattleen_GB
dc.subjectGene expressionen_GB
dc.subjectCompensatory growth (CG)en_GB
dc.subjectruminal epitheliumen_GB
dc.subjectimmune responseen_GB
dc.titleEffect of dietary restriction and subsequent re-alimentation on the transcriptional profile of bovine ruminal epitheliumen_GB
dc.typeArticleen_GB
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0177852
dc.contributor.sponsorScience Foundation Ireland
dc.contributor.sponsorGrantNumberRFP/GEN2447
refterms.dateFOA2018-01-12T08:57:36Z


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