Simulated gastrointestinal digestion of nisin and interaction between nisin and bile
Author
Gough, RonanO'Connor, Paula M.
Rea, Mary

Gomez-Sala, Beatriz
Miao, Song

Hill, Colin
Brodkorb, Andre

Date
14/08/2017
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Ronan Gough, Paula M. O'Connor, Mary C. Rea, Beatriz Gómez-Sala, Song Miao, Colin Hill, André Brodkorb, Simulated gastrointestinal digestion of nisin and interaction between nisin and bile, LWT - Food Science and Technology, 2017, 86, 530, DOI 10.1016/j.lwt.2017.08.031Abstract
Nisin, an antimicrobial peptide showing activity against many Gram positive bacteria, is widely used as a food preservative. The simulated gastrointestinal digestion of nisin (variant A) was studied using the in vitro INFOGEST digestion method. Following oral, gastric and small intestinal digestion, there was no intact nisin in the system and the nisin was primarily digested by pancreatin. After digestion, six nisin fragments (1–11, 1–12, 1–20, 1–21, 1–29 and 1–32) were identified by reversed phase high performance liquid chromatography and mass spectroscopy and four of these nisin fragments (1–20, 1–21, 1–29 and 1–32) demonstrated low antibacterial activity against Lactococcus lactis HP in agar diffusion activity assays. Additionally, it was observed that bile salts form a complex with nisin. This was examined by atomic force microscopy, turbidity and dynamic light scattering, which showed that this interaction resulted in significantly larger bile salt micelles. The presence of bile salts at physiological levels significantly altered the relative amounts of the nisin fragments 1–12, 1–20 and 1–29 produced during an in vitro digestion. This study highlights the importance of including bile in simulated digestions of antimicrobial peptides in order to obtain a more accurate simulation of the in vivo digestion products and their activity.Funder
Department of Agriculture, Food and the Marine; Teagasc Walsh Fellowship ProgrammeGrant Number
10/RD/TMFRC/701; 2012221ae974a485f413a2113503eed53cd6c53
https://doi.org/10.1016/j.lwt.2017.08.031