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    Insights into the Mode of Action of the Sactibiotic Thuricin CD

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    Author
    Mathur, Harsh
    Fallico, Vincenzo
    O'Connor, Paula M.
    Rea, Mary cc
    Cotter, Paul D.
    Hill, Colin
    Ross, R Paul
    Keyword
    Sactibiotic bacteriocins
    Thuricin CD
    Mode of Action
    Bacillus firmus DPC6349
    Lytic activity
    Date
    20/04/2017
    
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    URI
    http://hdl.handle.net/11019/1522
    Citation
    Mathur H, Fallico V, O’Connor PM, Rea MC, Cotter PD, Hill C and Ross RP (2017) Insights into the Mode of Action of the Sactibiotic Thuricin CD. Front. Microbiol. 8:696. doi: 10.3389/fmicb.2017.00696
    Abstract
    Thuricin CD is a two-component bacteriocin, consisting of the peptides Trnα and Trnβ, and belongs to the newly designated sactibiotic subclass of bacteriocins. While it is clear from studies conducted thus far that it is a narrow-spectrum bacteriocin, requiring the synergistic activity of the two peptides, the precise mechanism of action of thuricin CD has not been elucidated. This study used a combination of flow cytometry and traditional culture-dependent assays to ascertain the effects of the thuricin CD peptides on the morphology, physiology and viability of sensitive Bacillus firmus DPC6349 cells. We show that both Trnα and Trnβ are membrane-acting and cause a collapse of the membrane potential, which could not be reversed even under membrane-repolarizing conditions. Furthermore, the depolarizing action of thuricin CD is accompanied by reductions in cell size and granularity, producing a pattern of physiological alterations in DPC6349 cells similar to those triggered by the pore-forming single-component bacteriocin Nisin A, and two-component lacticin 3147. Taken together, these results lead us to postulate that the lytic activity of thuricin CD involves the insertion of thuricin CD peptides into the membrane of target cells leading to permeabilization due to pore formation and consequent flux of ions across the membrane, resulting in membrane depolarization and eventual cell death.
    Funder
    Science Foundation Ireland
    Grant Number
    SFI/12/RC/2273
    ae974a485f413a2113503eed53cd6c53
    https://doi.org/10.3389/fmicb.2017.00696
    Scopus Count
    Collections
    Food Biosciences
    Food Biosciences
    Food Biosciences
    Food Biosciences

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