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dc.contributor.authorArboleya, Silvia
dc.contributor.authorBottacini, Francesca
dc.contributor.authorO’Connell-Motherway, Mary
dc.contributor.authorRyan, C. A
dc.contributor.authorRoss, R Paul
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.authorStanton, Catherine
dc.date.accessioned2018-04-18T15:33:14Z
dc.date.available2018-04-18T15:33:14Z
dc.date.issued08/01/2018
dc.identifier.citationSilvia Arboleya;Francesca Bottacini;Mary O’Connell-Motherway;C. Anthony Ryan;R. Paul Ross;Douwe van Sinderen;Catherine Stanton. Gene-trait matching across the Bifidobacterium longum pan-genome reveals considerable diversity in carbohydrate catabolism among human infant strains. BMC Genomics. 2018 Jan 08;19(1):33. DOI: 10.1186/s12864-017-4388-9en_US
dc.identifier.urihttp://hdl.handle.net/11019/1534
dc.descriptionpeer-revieweden_US
dc.descriptionThis work was supported by The APC Microbiome Institute (under Science Foundation Ireland [SFI] grant number: SFI/12/RC/2273) and by Department of Agriculture Food and Marine (DAFM) funded INFANTMET (Infant Nutrition for Programming the Gut Microbiota in Neonates) Project (Ref No. 10FDairy).en_US
dc.description.abstractBackground Bifidobacterium longum is a common member of the human gut microbiota and is frequently present at high numbers in the gut microbiota of humans throughout life, thus indicative of a close symbiotic host-microbe relationship. Different mechanisms may be responsible for the high competitiveness of this taxon in its human host to allow stable establishment in the complex and dynamic intestinal microbiota environment. The objective of this study was to assess the genetic and metabolic diversity in a set of 20 B. longum strains, most of which had previously been isolated from infants, by performing whole genome sequencing and comparative analysis, and to analyse their carbohydrate utilization abilities using a gene-trait matching approach. Results We analysed their pan-genome and their phylogenetic relatedness. All strains clustered in the B. longum ssp. longum phylogenetic subgroup, except for one individual strain which was found to cluster in the B. longum ssp. suis phylogenetic group. The examined strains exhibit genomic diversity, while they also varied in their sugar utilization profiles. This allowed us to perform a gene-trait matching exercise enabling the identification of five gene clusters involved in the utilization of xylo-oligosaccharides, arabinan, arabinoxylan, galactan and fucosyllactose, the latter of which is an abundant human milk oligosaccharide (HMO). Conclusions The results showed high diversity in terms of genes and predicted glycosyl-hydrolases, as well as the ability to metabolize a large range of sugars. Moreover, we corroborate the capability of B. longum ssp. longum to metabolise HMOs. Ultimately, their intraspecific genomic diversity and the ability to consume a wide assortment of carbohydrates, ranging from plant-derived carbohydrates to HMOs, may provide an explanation for the competitive advantage and persistence of B. longum in the human gut microbiome.en_US
dc.language.isoenen_US
dc.publisherBiomed Centralen_US
dc.relation.ispartofseriesBMC Genomics;vol 19
dc.subjectBifidobacterium longum pan-genomeen_US
dc.subjectcarbohydrate catabolismen_US
dc.subjectInfantsen_US
dc.subjectGastrointestinal microbiotaen_US
dc.subjectGenetic diversityen_US
dc.titleGene-trait matching across the Bifidobacterium longum pan-genome reveals considerable diversity in carbohydrate catabolism among human infant strainsen_US
dc.typeArticleen_US
dc.date.updated2018-04-18T11:23:03Z
dc.language.rfc3066en
dc.rights.holderThe Author(s).
dc.identifier.doihttps://doi.org/10.1186/s12864-017-4388-9
dc.contributor.sponsorScience Foundation Irelanden_US
dc.contributor.sponsorDepartment of Agriculture, Food and the Marine, Irelanden_US
refterms.dateFOA2018-04-18T15:33:14Z
dc.contributor.sponsorGrrantNumberSFI/12/RC/2273en_US
dc.contributor.sponsorGrrantNumber10FDairyen_US


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