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dc.contributor.authorLafarga, Tomas*
dc.contributor.authorHayes, Maria*
dc.date.accessioned2018-07-09T11:56:12Z
dc.date.available2018-07-09T11:56:12Z
dc.date.issued25/05/2017
dc.identifier.citationLafarga, T., & Hayes, M. (2017). Effect of pre-treatment on the generation of dipeptidyl peptidase-IV- and prolyl endopeptidase-inhibitory hydrolysates from bovine lung, Irish Journal of Agricultural and Food Research, 56(1), 12-24. doi: https://doi.org/10.1515/ijafr-2017-0002en_US
dc.identifier.issn2009-9029
dc.identifier.issn0791-6833 (print)
dc.identifier.urihttp://hdl.handle.net/11019/1555
dc.descriptionpeer-revieweden_US
dc.description.abstractThe aim of this work was to study the effect of two different pre-treatments, high temperature (100 °C, 5 min) and high pressure (600 MPa, 3 min), on the potential of the enzymes papain, collagenase and Alcalase® to generate bioactive hydrolysates containing dipeptidyl peptidase-IV- (DPP-IV; EC 3.4.14.5) and prolyl endopeptidase- (PEP; EC 3.4.21.26) inhibitory peptides from bovine lung. Both pre-treatments resulted in an increase in the degree of hydrolysis over a 24 h period (P < 0.001) and significantly increased the DPP-IV- and PEP-inhibitory activities of the generated hydrolysates (P < 0.001). Generated hydrolysates included an Alcalase hydrolysate of pressure-treated bovine lung, which was the most active, and showed DPP-IV and PEP half-maximal inhibitory concentration (IC50) values of 1.43 ± 0.06 and 3.62 ± 0.07 mg/ mL, respectively. The major peptides contained in this hydrolysate were determined by liquid chromatography-tandem mass spectrometry, and results demonstrated that bovine lung is a good substrate for the release of bioactive peptides when proper pre-treatment and enzymatic treatment are applied.en_US
dc.language.isoenen_US
dc.publisherTeagasc (Agriculture and Food Development Authority), Irelanden_US
dc.relation.ispartofseriesIrish Journal of Agricultural and Food Research;vol 56
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectBioactive peptidesen_US
dc.subjectBovine lungen_US
dc.subjectDPP-IVen_US
dc.subjectHigh pressureen_US
dc.subjectPre-treatmenten_US
dc.titleEffect of pre-treatment on the generation of dipeptidyl peptidase-IV- and prolyl endopeptidase-inhibitory hydrolysates from bovine lungen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1515/ijafr-2017-0002
dc.contributor.sponsorDepartment of Agriculture, Food and the Marine, Irelanden_US
dc.contributor.sponsorTeagasc Walsh Fellowship Programmeen_US
dc.contributor.sponsorGrantNumber11/F/043en_US
refterms.dateFOA2018-07-09T11:56:12Z


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