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dc.contributor.authorLynch, Sarah A.*
dc.contributor.authorÁlvarez García, Carlos*
dc.contributor.authorO'Neill, Eileen*
dc.contributor.authorKeenan, Derek F.*
dc.contributor.authorMullen, Anne Maria*
dc.identifier.citationLynch, S. A., Álvarez, C. , O'Neill, E. E., Keenan, D. F. and Mullen, A. M. (2018), Optimization of protein recovery from bovine lung by pH shift process using response surface methodology. J. Sci. Food Agric, 98: 1951-1960. doi:10.1002/jsfa.8678en_US
dc.description.abstractBACKGROUND Response surface methodology (RSM) was used in a sequential manner to optimize solubilization and precipitation conditions in the recovery of protein from bovine lung using pH shift. RESULTS Separate D‐optimal designs were employed for protein solubilization and precipitation. Independent variables investigated for protein solubilization were time (10–120 min), temperature (4–20 °C), pH (8.0–11.0) and solvent/sample ratio (2.5–10). Variables for protein precipitation were time (0–60 min) and pH (4.25–6.00). Soluble protein yields ranged from 323 to 649 g kg−1 and the quadratic model for protein solubilization revealed a coefficient of determination R2 of 0.9958. Optimal conditions for maximum protein solubility were extraction time 140 min, temperature 19 °C, pH 10.8 and solvent/sample ratio 13.02. Protein precipitation yields varied from 407 to 667 g kg−1, giving a coefficient of determination R2 of 0.9335. Optimal conditions for maximum protein precipitation were pH 5.03 and 60 min. Based on the RSM model, solubilization conditions were manipulated to maximize protein solubilization under reduced water and alkaline usage. These conditions were also validated. CONCLUSION Models for solubilization and precipitation using bovine and porcine lung were validated; predicted and actual yields were in good agreement, showing cross‐species applicability of the results. © 2017 Society of Chemical Industryen_US
dc.relation.ispartofseriesJournal of the Science of Food and Agriculture;vol 98
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.subjectProtein solubilizationen_US
dc.subjectProtein precipitationen_US
dc.subjectpH Shiften_US
dc.subjectBovine offalen_US
dc.subjectResponse surface methodologyen_US
dc.titleOptimization of protein recovery from bovine lung by pH shift process using response surface methodologyen_US
dc.contributor.sponsorDepartment of Agriculture, Food and the Marine, Irelanden_US
dc.contributor.sponsorTeagasc Walsh Fellowship Programmeen_US

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