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    Improved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattle

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    Author
    Adnane, Mounir
    Kelly, Paul M
    Chapwanya, Aspinas
    Meade, Kieran G
    O'Farrelly, Cliona
    Keyword
    Cervico-vaginal mucus
    Postpartum
    Reducing agent
    Endometritis
    Biomarkers
    Date
    2018-09-29
    
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    URI
    http://hdl.handle.net/11019/1603
    Citation
    Adnane M, Kelly P, Chapwanya A, Meade KG, O’Farrelly C. Improved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattle. BMC Veterinary Research 2018;14(1):297; doi 10.1186/s12917-018-1619-5
    Abstract
    Background In the postpartum cow, early diagnosis of uterine disease is currently problematic due to the lack of reliable, non-invasive diagnostic methods. Cervico-vaginal mucus (CVM) is an easy to collect potentially informative source of biomarkers for the diagnosis and prognosis of uterine disease in cows. Here, we report an improved method for processing CVM from postpartum dairy cows for the measurement of immune biomarkers. CVM samples were collected from the vagina using gloved hand during the first two weeks postpartum and processed with buffer alone or buffer containing different concentrations of the reducing agents recommended in standard protocols: Dithiothriotol (DTT) or N-Acetyl-L-Cysteine (NAC). Total protein was measured using the bicinchoninic acid (BCA) assay; interleukin 6 (IL-6), IL-8 and α1-acid glycoprotein (AGP) were measured by ELISA. Results We found that use of reducing agents to liquefy CVM affects protein yield and the accuracy of biomarker detection. Our improved protocol results in lower protein yields but improved detection of cytokines and chemokines. Using our modified method to measure AGP in CVM we found raised levels of AGP at seven days postpartum in CVM from cows that went on to develop endometritis. Conclusion We conclude that processing CVM without reducing agents improves detection of biomarkers that reflect uterine health in cattle. We propose that measurement of AGP in CVM during the first week postpartum may identify cows at risk of developing clinical endometritis.
    Funder
    The Algerian Ministry for High Education and Scientific Research; University of Tiaret, Algeria; Department of Agriculture, Food and the Marine, Ireland
    Grant Number
    ofarrecl-HRB-HRA_POR/2012/37; 13/S/472
    ae974a485f413a2113503eed53cd6c53
    https://doi.org/10.1186/s12917-018-1619-5
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    Animal & Bioscience
    Teagasc publications in Biomed Central

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