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dc.contributor.authorAdnane, Mounir*
dc.contributor.authorKelly, Paul M*
dc.contributor.authorChapwanya, Aspinas*
dc.contributor.authorMeade, Kieran G*
dc.contributor.authorO'Farrelly, Cliona*
dc.date.accessioned2018-10-11T11:29:50Z
dc.date.available2018-10-11T11:29:50Z
dc.date.issued2018-09-29
dc.identifier.citationAdnane M, Kelly P, Chapwanya A, Meade KG, O’Farrelly C. Improved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattle. BMC Veterinary Research 2018;14(1):297; doi 10.1186/s12917-018-1619-5en_US
dc.identifier.urihttp://hdl.handle.net/11019/1603
dc.descriptionpeer-revieweden_US
dc.description.abstractBackground In the postpartum cow, early diagnosis of uterine disease is currently problematic due to the lack of reliable, non-invasive diagnostic methods. Cervico-vaginal mucus (CVM) is an easy to collect potentially informative source of biomarkers for the diagnosis and prognosis of uterine disease in cows. Here, we report an improved method for processing CVM from postpartum dairy cows for the measurement of immune biomarkers. CVM samples were collected from the vagina using gloved hand during the first two weeks postpartum and processed with buffer alone or buffer containing different concentrations of the reducing agents recommended in standard protocols: Dithiothriotol (DTT) or N-Acetyl-L-Cysteine (NAC). Total protein was measured using the bicinchoninic acid (BCA) assay; interleukin 6 (IL-6), IL-8 and α1-acid glycoprotein (AGP) were measured by ELISA. Results We found that use of reducing agents to liquefy CVM affects protein yield and the accuracy of biomarker detection. Our improved protocol results in lower protein yields but improved detection of cytokines and chemokines. Using our modified method to measure AGP in CVM we found raised levels of AGP at seven days postpartum in CVM from cows that went on to develop endometritis. Conclusion We conclude that processing CVM without reducing agents improves detection of biomarkers that reflect uterine health in cattle. We propose that measurement of AGP in CVM during the first week postpartum may identify cows at risk of developing clinical endometritis.en_US
dc.language.isoenen_US
dc.publisherBiomed Centralen_US
dc.relation.ispartofseriesBMC Veterinary Research;vol 14
dc.subjectCervico-vaginal mucusen_US
dc.subjectPostpartumen_US
dc.subjectReducing agenten_US
dc.subjectEndometritisen_US
dc.subjectBiomarkersen_US
dc.titleImproved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattleen_US
dc.typeArticleen_US
dc.date.updated2018-09-30T03:55:03Z
dc.language.rfc3066en
dc.rights.holderThe Author(s).
dc.identifier.doihttps://doi.org/10.1186/s12917-018-1619-5
dc.contributor.sponsorThe Algerian Ministry for High Education and Scientific Researchen_US
dc.contributor.sponsorUniversity of Tiaret, Algeriaen_US
dc.contributor.sponsorDepartment of Agriculture, Food and the Marine, Irelanden_US
dc.contributor.sponsorGrantNumberofarrecl-HRB-HRA_POR/2012/37en_US
dc.contributor.sponsorGrantNumber13/S/472en_US
refterms.dateFOA2018-10-11T11:29:50Z


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