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dc.contributor.authorThorogood, Daniel*
dc.contributor.authorYates, Steven*
dc.contributor.authorManzanares, Chloé*
dc.contributor.authorSkot, Leif*
dc.contributor.authorHegarty, Matthew*
dc.contributor.authorBlackmore, Tina*
dc.contributor.authorBarth, Susanne*
dc.contributor.authorStuder, Bruno*
dc.date.accessioned2018-12-07T12:39:07Z
dc.date.available2018-12-07T12:39:07Z
dc.date.issued2017-08-02
dc.identifier.citationThorogood, D., Yates, S., Manzanares, C., Skot, L., Hegarty, M., Blackmore, T., Barth, S., and Studer, B. (2017). A Novel Multivariate Approach to Phenotyping and Association Mapping of Multi-Locus Gametophytic Self-Incompatibility Reveals S, Z, and Other Loci in a Perennial Ryegrass (Poaceae) Population. Frontiers in Plant Science 8:1331. DOI: https://doi.org/10.3389/fpls.2017.01331en_US
dc.identifier.urihttp://hdl.handle.net/11019/1640
dc.descriptionpeer-revieweden_US
dc.description.abstractSelf-incompatibility (SI) is a mechanism that many flowering plants employ to prevent fertilisation by self- and self-like pollen ensuring heterozygosity and hybrid vigour. Although a number of single locus mechanisms have been characterised in detail, no multi-locus systems have been fully elucidated. Historically, examples of the genetic analysis of multi-locus SI, to make analysis tractable, are either made on the progeny of bi-parental crosses, where the number of alleles at each locus is restricted, or on crosses prepared in such a way that only one of the SI loci segregates. Perennial ryegrass (Lolium perenne L.) possesses a well-documented two locus (S and Z) gametophytic incompatibility system. A more universal, realistic proof of principle study was conducted in a perennial ryegrass population in which allelic and non-allelic diversity was not artificially restricted. A complex pattern of pollinations from a diallel cross was revealed which could not possibly be interpreted easily per se, even with an already established genetic model. Instead, pollination scores were distilled into principal component scores described as Compatibility Components (CC1-CC3). These were then subjected to a conventional genome-wide association analysis. CC1 associated with markers on linkage groups (LGs) 1, 2, 3, and 6, CC2 exclusively with markers in a genomic region on LG 2, and CC3 with markers on LG 1. BLAST alignment with the Brachypodium physical map revealed highly significantly associated markers with peak associations with genes adjacent and four genes away from the chromosomal locations of candidate SI genes, S- and Z-DUF247, respectively. Further significant associations were found in a Brachypodium distachyon chromosome 3 region, having shared synteny with Lolium LG 1, suggesting further SI loci linked to S or extensive micro-re-arrangement of the genome between B. distachyon and L. perenne. Significant associations with gene sequences aligning with marker sequences on Lolium LGs 3 and 6 were also identified. We therefore demonstrate the power of a novel association genetics approach to identify the genes controlling multi-locus gametophytic SI systems and to identify novel loci potentially involved in already established SI systems.en_US
dc.language.isoenen_US
dc.publisherFrontiersen_US
dc.relation.ispartofseriesFrontiers in Plant Science;
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectDUF247en_US
dc.subjectgametophyticen_US
dc.subjectgenome wide association studies (GWAS)en_US
dc.subjectprincipal components analysis (PCA)en_US
dc.subjectpollen-stigma incompatibilityen_US
dc.subjectS-locusen_US
dc.subjectself-incompatibility (SI)en_US
dc.subjectZ-locusen_US
dc.titleA Novel Multivariate Approach to Phenotyping and Association Mapping of Multi-Locus Gametophytic Self-Incompatibility Reveals S, Z, and Other Loci in a Perennial Ryegrass (Poaceae) Populationen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.3389/fpls.2017.01331
dc.contributor.sponsorBiotechnology and Biological Sciences Research Councilen_US
dc.contributor.sponsorSwiss National Science Foundationen_US
dc.contributor.sponsorTeagasc Walsh Fellowship Programmeen_US
dc.contributor.sponsorGrantNumberBB/J004405/1en_US
dc.contributor.sponsorGrantNumberPP00P2 138988en_US
refterms.dateFOA2018-12-07T12:39:07Z


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