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dc.contributor.authorVelmurugan, Janaki
dc.contributor.authorMilbourne, Dan
dc.contributor.authorConnolly, Vincent
dc.contributor.authorHeslop-Harrison, J. S.
dc.contributor.authorAnhalt, Ulrike C. M.
dc.contributor.authorLynch, M. B.
dc.contributor.authorBarth, Susanne
dc.date.accessioned2018-12-10T12:48:07Z
dc.date.available2018-12-10T12:48:07Z
dc.date.issued2018-05-31
dc.identifier.citationVelmurugan, J., Milbourne, D., Connolly, V., Heslop-Harrison, J. S., Anhalt, U. C. M., Lynch, M. B., and Barth, S. (2018). An Immortalized Genetic Mapping Population for Perennial Ryegrass: A Resource for Phenotyping and Complex Trait Mapping. Frontiers in Plant Science 9(717). https://doi.org/10.3389/fpls.2018.00717en_US
dc.identifier.urihttp://hdl.handle.net/11019/1644
dc.descriptionpeer-revieweden_US
dc.description.abstractTo address the lack of a truly portable, universal reference mapping population for perennial ryegrass, we have been developing a recombinant inbred line (RIL) mapping population of perennial ryegrass derived via single seed descent from a well-characterized F2 mapping population based on genetically distinct inbred parents in which the natural self-incompatibility (SI) system of perennial ryegrass has been overcome. We examined whether it is possible to create a genotyping by sequencing (GBS) based genetic linkage map in a small population of the F6 generation of this population. We used 41 F6 genotypes for GBS with PstI/MspI-based libraries. We successfully developed a genetic linkage map comprising 6074 SNP markers, placing a further 22080 presence and absence variation (PAV) markers on the map. We examined the resulting genetic map for general and RIL specific features. Overall segregation distortion levels were similar to those experienced in the F2 generation, but segregation distortion was reduced on linkage group 6 and increased on linkage group 7. Residual heterozygosity in the F6 generation was observed at a level of 5.4%. There was a high proportion of chromosomes (30%) exhibiting the intact haplotype of the original inbred parents of the F1 genotype from which the population is derived, pointing to a tendency for chromosomes to assort without recombining. This could affect the applicability of these lines and might make them more suitable for situations where repressed recombination is an advantage. Inter- and intra-chromosomal linkage disequilibrium (LD) analysis suggested that the map order was robust. We conclude that this RIL population, and subsequent F7 and F8 generations will be useful for genetic analysis and phenotyping of agronomic and biological important traits in perennial ryegrass.en_US
dc.language.isoenen_US
dc.publisherFrontiersen_US
dc.relation.ispartofseriesFrontiers in Plant Science;
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectperennial ryegrassen_US
dc.subjectLolium perenneen_US
dc.subjectrecombinant inbred lines (RIL)en_US
dc.subjectgenotyping by sequencingen_US
dc.subjectmapping populationen_US
dc.subjectphenotypingen_US
dc.titleAn Immortalized Genetic Mapping Population for Perennial Ryegrass: A Resource for Phenotyping and Complex Trait Mappingen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.3389/fpls.2018.00717
dc.contributor.sponsorTeagasc Walsh Fellowship Programmeen_US
refterms.dateFOA2018-12-10T12:48:08Z


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