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dc.contributor.authorHeras, Vanessa L*
dc.contributor.authorClooney, Adam G*
dc.contributor.authorRyan, Feargal J*
dc.contributor.authorCabrera-Rubio, Raul*
dc.contributor.authorCasey, Patrick G.*
dc.contributor.authorHueston, Cara M*
dc.contributor.authorPinheiro, Jorge*
dc.contributor.authorRudkin, Justine K*
dc.contributor.authorMelgar, Silvia*
dc.contributor.authorCotter, Paul D.*
dc.contributor.authorHill, Colin*
dc.contributor.authorGahan, Cormac G M*
dc.date.accessioned2019-06-11T16:07:21Z
dc.date.available2019-06-11T16:07:21Z
dc.date.issued2019-01-18
dc.identifier.citationLas Heras V, Clooney AG, Ryan FJ, Cabrera-Rubio R, Casey PG, Hueston CM, Pinheiro J, Rudkin JK, Melgar S, Cotter PD and others. Short-term consumption of a high-fat diet increases host susceptibility to Listeria monocytogenes infection. Microbiome 2019;7(1):7; doi http://dx.doi.org/10.1186/s40168-019-0621-x.en_US
dc.identifier.urihttp://hdl.handle.net/11019/1665
dc.descriptionpeer-revieweden_US
dc.description.abstractBackground A westernized diet comprising a high caloric intake from animal fats is known to influence the development of pathological inflammatory conditions. However, there has been relatively little focus upon the implications of such diets for the progression of infectious disease. Here, we investigated the influence of a high-fat (HF) diet upon parameters that influence Listeria monocytogenes infection in mice. Results We determined that short-term administration of a HF diet increases the number of goblet cells, a known binding site for the pathogen, in the gut and also induces profound changes to the microbiota and promotes a pro-inflammatory gene expression profile in the host. Host physiological changes were concordant with significantly increased susceptibility to oral L. monocytogenes infection in mice fed a HF diet relative to low fat (LF)- or chow-fed animals. Prior to Listeria infection, short-term consumption of HF diet elevated levels of Firmicutes including Coprococcus, Butyricicoccus, Turicibacter and Clostridium XIVa species. During active infection with L. monocytogenes, microbiota changes were further exaggerated but host inflammatory responses were significantly downregulated relative to Listeria-infected LF- or chow-fed groups, suggestive of a profound tempering of the host response influenced by infection in the context of a HF diet. The effects of diet were seen beyond the gut, as a HF diet also increased the sensitivity of mice to systemic infection and altered gene expression profiles in the liver. Conclusions We adopted a systems approach to identify the effects of HF diet upon L. monocytogenes infection through analysis of host responses and microbiota changes (both pre- and post-infection). Overall, the results indicate that short-term consumption of a westernized diet has the capacity to significantly alter host susceptibility to L. monocytogenes infection concomitant with changes to the host physiological landscape. The findings suggest that diet should be a consideration when developing models that reflect human infectious disease.en_US
dc.description.sponsorshipThis research was funded by the European Union’s Horizon 2020 Research and Innovation Program under the Marie Skłodowska-Curie grant agreement No. 641984, through funding of the List_MAPS consortium. We also acknowledge funding and support from Science Foundation Ireland (SFI) in the form of a center grant (APC Microbiome Ireland grant SFI/12/RC/2273).
dc.language.isoenen_US
dc.publisherBiomed Centralen_US
dc.relation.ispartofseriesMicrobiome;vol 7
dc.subjectDieten_US
dc.subjectInfectionen_US
dc.subjectMicrobiomeen_US
dc.subjectListeria monocytogenesen_US
dc.subjectMiceen_US
dc.subjectMurineen_US
dc.subjectImmunityen_US
dc.subjectGoblet cellen_US
dc.titleShort-term consumption of a high-fat diet increases host susceptibility to Listeria monocytogenes infectionen_US
dc.typeArticleen_US
dc.date.updated2019-01-20T04:16:12Z
dc.language.rfc3066en
dc.rights.holderThe Author(s).
dc.identifier.doihttps://doi.org/10.1186/s40168-019-0621-x
dc.contributor.sponsorEuropean Unionen_US
dc.contributor.sponsorScience Foundation Irelanden_US
dc.contributor.sponsorGrantNumber641984en_US
dc.contributor.sponsorGrantNumberSFI/12/RC/2273en_US
refterms.dateFOA2019-06-11T16:07:21Z


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