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dc.contributor.authorJiang, Yan*
dc.contributor.authorDennehy, Conor*
dc.contributor.authorLawlor, Peadar G*
dc.contributor.authorHu, Zhenhu*
dc.contributor.authorMcCabe, Matthew*
dc.contributor.authorCormican, Paul*
dc.contributor.authorZhan, Xinmin*
dc.contributor.authorGardiner, Gillian E*
dc.date.accessioned2019-06-11T16:18:23Z
dc.date.available2019-06-11T16:18:23Z
dc.date.issued2019-01-04
dc.identifier.citationJiang Y, Dennehy C, Lawlor PG, Hu Z, McCabe M, Cormican P, Zhan X, Gardiner GE. Exploring the roles of and interactions among microbes in dry co-digestion of food waste and pig manure using high-throughput 16S rRNA gene amplicon sequencing. Biotechnology for Biofuels 2019;12(1):5; doi http://dx.doi.org/10.1186/s13068-018-1344-0.en_US
dc.identifier.urihttp://hdl.handle.net/11019/1667
dc.descriptionpeer-revieweden_US
dc.description.abstractBackground With the increasing global population and increasing demand for food, the generation of food waste and animal manure increases. Anaerobic digestion is one of the best available technologies for food waste and pig manure management by producing methane-rich biogas. Dry co-digestion of food waste and pig manure can significantly reduce the reactor volume, capital cost, heating energy consumption and the cost of digestate liquid management. It is advantageous over mono-digestion of food waste or pig manure due to the balanced carbon/nitrogen ratio, high pH buffering capacity, and provision of trace elements. However, few studies have been carried out to study the roles of and interactions among microbes in dry anaerobic co-digestion systems. Therefore, this study aimed to assess the effects of different inocula (finished digestate and anaerobic sludge taken from wastewater treatment plants) and substrate compositions (food waste to pig manure ratios of 50:50 and 75:25 in terms of volatile solids) on the microbial community structure in food waste and pig manure dry co-digestion systems, and to examine the possible roles of the previously poorly described bacteria and the interactions among dry co-digestion-associated microbes. Results The dry co-digestion experiment lasted for 120 days. The microbial profile during different anaerobic digestion stages was explored using high-throughput 16S rRNA gene amplicon sequencing. It was found that the inoculum factor was more significant in determining the microbial community structure than the substrate composition factor. Significant correlation was observed between the relative abundance of specific microbial taxa and digesters’ physicochemical parameters. Hydrogenotrophic methanogens dominated in dry co-digestion systems. Conclusions The possible roles of specific microbial taxa were explored by correlation analysis, which were consistent with the literature. Based on this, the anaerobic digestion-associated roles of 11 bacteria, which were previously poorly understood, were estimated here for the first time. The inoculum played a more important role in determining the microbial community structure than substrate composition in dry co-digestion systems. Hydrogenotrophic methanogenesis was a significant methane production pathway in dry co-digestion systems.en_US
dc.language.isoenen_US
dc.publisherBiomed Centralen_US
dc.relation.ispartofseriesBiotechnology for Biofuels;vol 12
dc.subjectCo-digestionen_US
dc.subjectCorrelation analysisen_US
dc.subjectDry digestionen_US
dc.subjectFood wasteen_US
dc.subjectHydrogenotrophic methanogenesisen_US
dc.subjectInoculumen_US
dc.subjectPig manureen_US
dc.subjectSubstrateen_US
dc.subjectSyntrophic oxidationen_US
dc.titleExploring the roles of and interactions among microbes in dry co-digestion of food waste and pig manure using high-throughput 16S rRNA gene amplicon sequencingen_US
dc.typeArticleen_US
dc.date.updated2019-01-06T04:13:50Z
dc.language.rfc3066en
dc.rights.holderThe Author(s)
dc.identifier.doihttps://doi.org/10.1186/s13068-018-1344-0
dc.contributor.sponsorGreen Farm projecten_US
dc.contributor.sponsorNatural Science Foundation of Chinaen_US
dc.contributor.sponsorGrantNumber12/IP/1519en_US
dc.contributor.sponsorGrantNumber51728801)en_US
refterms.dateFOA2019-06-11T16:18:24Z


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