INFOGEST static in vitro simulation of gastrointestinal food digestion
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Brodkorb, AndreEgger, Lotti
Alminger, Marie
Alvito, Paula
Assuncao, Ricardo
Balance, Simon
Bohn, Torsten
Bourlieu-Lacanal, Claire
Boutrou, Rachel
Carriere, Frederic
Clemente, Alfonso
Corredig, Milena
Dupont, Didier
Dufour, Claire
Edwards, Cathrina
Golding, Matt
Karakaya, Sibel
Kirkhus, Bente
Le Feunteun, Steven
Lesmes, Uri
Macierzanka, Adam
Mackie, Alan R.
Martins, Carla
Marze, Sebastien
McClements, David J.
Menard, Olivia
Minekus, Mans
Portmann, Reto
Santos, Claudia N.
Souchon, Isabelle
Singh, R. Paul
Vegarud, Gerd E.
Wickham, Martin S. J.
Weitschies, Werner
Recio, Isidra
Date
2019-03-18
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Brodkorb A, Egger L, Alminger M, Alvito P, Assunção R, Ballance S, Bohn T, Bourlieu-Lacanal C, Boutrou R, Carrière F and others. INFOGEST static in vitro simulation of gastrointestinal food digestion. Nature Protocols 2019;14(4):991-1014; doi http://dx.doi.org/10.1038/s41596-018-0119-1.Abstract
Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract. These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results. The standardized protocol presented here is based on an international consensus developed by the COST INFOGEST network. The method is designed to be used with standard laboratory equipment and requires limited experience to encourage a wide range of researchers to adopt it. It is a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion. This makes the method simple to use but not suitable for simulating digestion kinetics. Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data. This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase. The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix. The whole protocol can be completed in ~7 d, including ~5 d required for the determination of enzyme activities.Funder
French National Institute for Agricultural Research; European Cooperation in Science and Technology (COST)Grant Number
FA1005ae974a485f413a2113503eed53cd6c53
http://dx.doi.org/10.1038/s41596-018-0119-1
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