Show simple item record

dc.contributor.authorBrodkorb, Andre
dc.contributor.authorEgger, Lotti
dc.contributor.authorAlminger, Marie
dc.contributor.authoret al
dc.date.accessioned2019-06-25T09:17:45Z
dc.date.available2019-06-25T09:17:45Z
dc.date.issued2019-03-18
dc.identifier.citationBrodkorb A, Egger L, Alminger M, Alvito P, Assunção R, Ballance S, Bohn T, Bourlieu-Lacanal C, Boutrou R, Carrière F and others. INFOGEST static in vitro simulation of gastrointestinal food digestion. Nature Protocols 2019;14(4):991-1014; doi http://dx.doi.org/10.1038/s41596-018-0119-1.en_US
dc.identifier.urihttp://hdl.handle.net/11019/1671
dc.descriptionpeer-revieweden_US
dc.descriptionSupplementary information is available at http://dx.doi.org/10.1038/s41596-018-0119-1 or https://www.nature.com/articles/s41596-018-0119-1#Sec45.
dc.description.abstractDeveloping a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract. These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results. The standardized protocol presented here is based on an international consensus developed by the COST INFOGEST network. The method is designed to be used with standard laboratory equipment and requires limited experience to encourage a wide range of researchers to adopt it. It is a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion. This makes the method simple to use but not suitable for simulating digestion kinetics. Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data. This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase. The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix. The whole protocol can be completed in ~7 d, including ~5 d required for the determination of enzyme activities.en_US
dc.description.sponsorshipCOST action FA1005 INFOGEST (http://www.cost-infogest.eu/ ) is acknowledged for providing funding for travel, meetings and conferences (2011-2015). The French National Institute for Agricultural Research (INRA, www.inra.fr) is acknowledged for their continuous support of the INFOGEST network by organising and co-funding the International Conference on Food Digestion and workgroup meetings
dc.language.isoenen_US
dc.publisherNature Publishing Groupen_US
dc.relation.ispartofseriesNature Protocols;vol 14
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectstatic in vitro digestionen_US
dc.subjectfood digestionen_US
dc.subjectphysiological digestionen_US
dc.subjectsimulated digestionen_US
dc.titleINFOGEST static in vitro simulation of gastrointestinal food digestionen_US
dc.typeArticleen_US
dc.embargo.terms2019-09-18en_US
dc.identifier.doihttp://dx.doi.org/10.1038/s41596-018-0119-1
dc.contributor.sponsorFrench National Institute for Agricultural Researchen_US
dc.contributor.sponsorEuropean Cooperation in Science and Technology (COST)en_US
dc.contributor.sponsorGrantNumberFA1005en_US


Files in this item

Thumbnail
Name:
OPEN ACCESS word version Brodkorb ...
Embargo:
2019-09-18
Size:
2.253Mb
Format:
PDF
Description:
Main protocol

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-ShareAlike 3.0 United States
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 3.0 United States