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dc.contributor.authorDeng, Kaibo
dc.contributor.authorFang, Wei
dc.contributor.authorZheng, Baodong
dc.contributor.authorMiao, Song
dc.contributor.authorHuo, Guicheng
dc.date.accessioned2019-08-12T11:43:14Z
dc.date.available2019-08-12T11:43:14Z
dc.date.issued2017-12-21
dc.identifier.citationDeng, K., Fang, W., Zheng, B., Miao, S., & Huo, G. (2017). Phenotypic, fermentation characterization, and resistance mechanism analysis of bacteriophage-resistant mutants of Lactobacillus delbrueckii ssp. bulgaricus isolated from traditional Chinese dairy products. Journal of Dairy Science, 101(3), 1901–1914. https://doi.org/10.3168/jds.2017-13823en_US
dc.identifier.urihttp://hdl.handle.net/11019/1703
dc.descriptionpeer-revieweden_US
dc.description.abstractBacteriophage infection is a large factor in dairy industrial production failure on the basis of pure inoculation fermentation, and developing good commercial starter cultures from wild dairy products and improving the environmental vigor of starter cultures by enhancing their phage resistance are still the most effective solutions. Here we used a spontaneous isolation method to obtain bacteriophage-resistant mutants of Lactobacillus delbrueckii ssp. bulgaricus strains that are used in traditional Chinese fermented dairy products. We analyzed their phenotypes, fermentation characteristics, and resistance mechanisms. The results showed that bacteriophage-insensitive mutants (BIM) BIM8 and BIM12 had high bacteriophage resistance while exhibiting fermentation and coagulation attributes that were as satisfying as those of their respective parent strains KLDS1.1016 and KLDS1.1028. According to the attachment receptor detection, mutants BIM8 and BIM12 exhibited reduced absorption to bacteriophage phiLdb compared with their respective bacteriophage-sensitive parent strains because of changes to the polysaccharides or teichoic acids connected to their peptidoglycan layer. Additionally, genes, including HSDR, HSDM, and HSDS, encoding 3 subunits of a type I restriction-modification system were identified in their respective parent strains. We also discovered that HSDR and HSDM were highly conserved but that HSDS was variable because it is responsible for the DNA specificity of the complex. The late lysis that occurred only in strain KLDS1.1016 and not in strain KLDS1.1028 suggests that the former and its mutant BIM8 also may have an activatable restriction-modification mechanism. We conclude that the L. bulgaricus BIM8 and BIM12 mutants have great potential in the dairy industry as starter cultures, and their phage-resistance mechanism was effective mainly due to the adsorption interference and restriction-modification system.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofseriesJournal of Dairy Science;Vol. 101 (3)
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectLactobacillus delbrueckii ssp. bulgaricusen_US
dc.subjectstarter cultureen_US
dc.subjectbacteriophage-resistant mechanismen_US
dc.subjectspontaneous mutationen_US
dc.titlePhenotypic, fermentation characterization, and resistance mechanism analysis of bacteriophage-resistant mutants of Lactobacillus delbrueckii ssp. bulgaricus isolated from traditional Chinese dairy productsen_US
dc.typeArticleen_US
dc.embargo.terms2018-12-21en_US
dc.identifier.doihttps://dx.doi.org/10.3168/jds.2017-13823
dc.contributor.sponsorInternational Technological Cooperation and Exchange Plan of Fujian Agriculture and Forestry Universityen_US
dc.contributor.sponsorNational Natural Science Funds of Chinaen_US
dc.contributor.sponsorScientific and Technological Innovation Team Support Plan of Fujian Agriculture and Forestry Universityen_US
dc.contributor.sponsorGrantNumberKXGH17001en_US
dc.contributor.sponsorGrantNumber31171717en_US
dc.contributor.sponsorGrantNumberCXTD12009en_US
refterms.dateFOA2018-12-21T00:00:00Z


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