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dc.contributor.authorCollins, Fergus W. J.
dc.contributor.authorMesa-Pereira, Beatriz
dc.contributor.authorO'Connor, Paula M.
dc.contributor.authorRea, Mary C.
dc.contributor.authorHill, Colin
dc.contributor.authorRoss, R Paul
dc.date.accessioned2019-08-13T10:36:19Z
dc.date.available2019-08-13T10:36:19Z
dc.date.issued2018-07-02
dc.identifier.citationCollins FWJ, Mesa-Pereira B, O'Connor PM, Rea MC, Hill C and Ross RP (2018) Reincarnation of Bacteriocins From the Lactobacillus Pangenomic Graveyard. Front. Microbiol. 9:1298. doi: 10.3389/fmicb.2018.01298en_US
dc.identifier.urihttp://hdl.handle.net/11019/1731
dc.descriptionpeer-revieweden_US
dc.description.abstractBacteria commonly produce narrow spectrum bacteriocins as a means of inhibiting closely related species competing for similar resources in an environment. The increasing availability of genomic data means that it is becoming easier to identify bacteriocins encoded within genomes. Often, however, the presence of bacteriocin genes in a strain does not always translate into biological antimicrobial activity. For example, when analysing the Lactobacillus pangenome we identified strains encoding ten pediocin-like bacteriocin structural genes which failed to display inhibitory activity. Nine of these bacteriocins were novel whilst one was identified as the previously characterized bacteriocin “penocin A.” The composition of these bacteriocin operons varied between strains, often with key components missing which are required for bacteriocin production, such as dedicated bacteriocin transporters and accessory proteins. In an effort to functionally express these bacteriocins, the structural genes for the ten pediocin homologs were cloned alongside the dedicated pediocin PA-1 transporter in both Escherichia coli and Lactobacillus paracasei heterologous hosts. Each bacteriocin was cloned with its native leader sequence and as a fusion protein with the pediocin PA-1 leader sequence. Several of these bacteriocins displayed a broader spectrum of inhibition than the original pediocin PA-1. We show how potentially valuable bacteriocins can easily be “reincarnated” from in silico data and produced in vitro despite often lacking the necessary accompanying machinery. Moreover, the study demonstrates how genomic datasets such as the Lactobacilus pangenome harbor a potential “arsenal” of antimicrobial activity with the possibility of being activated when expressed in more genetically amenable hosts.en_US
dc.language.isoenen_US
dc.publisherFrontiersen_US
dc.relation.ispartofseriesFrontiers in Microbiology;
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectbacteriocinsen_US
dc.subjectpediocinen_US
dc.subjectheterologous expressionen_US
dc.subjectEscherichia colien_US
dc.subjectLactobacillusen_US
dc.titleReincarnation of Bacteriocins From the Lactobacillus Pangenomic Graveyarden_US
dc.typeArticleen_US
dc.identifier.doihttps://dx.doi.org/10.3389/fmicb.2018.01298
dc.contributor.sponsorScience Foundation Irelanden_US
dc.contributor.sponsorGrantNumberSFI/12/RC/227en_US
refterms.dateFOA2019-08-13T10:36:19Z


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