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dc.contributor.authorGough, Ronan
dc.contributor.authorCabrera-Rubio, Raúl
dc.contributor.authorO'Connor, Paula M.
dc.contributor.authorCrispie, Fiona
dc.contributor.authorBrodkorb, André
dc.contributor.authorMiao, Song
dc.contributor.authorHill, Colin
dc.contributor.authorRoss, Reynolds P.
dc.contributor.authorCotter, Paul D.
dc.contributor.authorNilaweera, Kanishka N.
dc.contributor.authorRea, Mary C.
dc.date.accessioned2019-08-13T11:00:51Z
dc.date.available2019-08-13T11:00:51Z
dc.date.issued2018-06-15
dc.identifier.citationGough R, Cabrera Rubio R, O'Connor PM, Crispie F, Brodkorb A, Miao S, Hill C, Ross RP, Cotter PD, Nilaweera KN and Rea MC (2018) Oral Delivery of Nisin in Resistant Starch Based Matrices Alters the Gut Microbiota in Mice. Front. Microbiol. 9:1186. doi: 10.3389/fmicb.2018.01186en_US
dc.identifier.urihttp://hdl.handle.net/11019/1732
dc.descriptionpeer-revieweden_US
dc.description.abstractThere is a growing recognition of the role the gastrointestinal microbiota plays in health and disease. Ingested antimicrobial proteins and peptides have the potential to alter the gastrointestinal microbiota; particularly if protected from digestion. Nisin is an antimicrobial peptide that is used as a food preservative. This study examined the ability of nisin to affect the murine microbiota when fed to mice in two different starch based matrices; a starch dough comprising raw starch granules and a starch gel comprising starch that was gelatinized and retrograded. The effects of the two starch matrices by themselves on the microbiota were also examined. Following 16S rRNA compositional sequencing, beta diversity analysis highlighted a significant difference (p = 0.001, n = 10) in the murine microbiota between the four diet groups. The differences between the two nisin containing diets were mainly attributable to differences in the nisin release from the starch matrices while the differences between the carriers were mainly attributable to the type of resistant starch they possessed. Indeed, the differences in the relative abundance of several genera in the mice consuming the starch dough and starch gel diets, in particular Akkermansia, the relative abundance of which was 0.5 and 11.9%, respectively (p = 0.0002, n = 10), points to the potential value of resistance starch as a modulator of beneficial gut microbes. Intact nisin and nisin digestion products (in particular nisin fragment 22–31) were detected in the feces and the nisin was biologically active. However, despite a three-fold greater consumption of nisin in the group fed the nisin in starch dough diet, twice as much nisin was detected in the feces of the group which consumed the nisin in starch gel diet. In addition, the relative abundance of three times as many genera from the lower gastrointestinal tract (GIT) were significantly different (p < 0.001, n = 10) to the control for the group fed the nisin in starch gel diet, implying that the starch gel afforded a degree of protection from digestion to the nisin entrapped within it.en_US
dc.description.sponsorshipThis work was funded by the Irish Department of Agriculture, Food and the Marine under the FIRM program (grant reference 10/RD/TMFRC/701). RG was funded under the Teagasc Walsh Fellowship scheme (grant reference 2012221). RC, PO, FC, AB, SM, CH, RR, PC, KN and MR were funded by Teagasc, Ireland. RC, PO, FC, CH, RR, and MR were supported in part by a research grant from Science Foundation Ireland (grant reference SFI/12/RC2273). KN was also funded by Science Foundation Ireland under the grant reference SFI/16/BBSRC/3389 and the UK Biotechnology and Biological Sciences Research Council under grant reference BB/P009875/1.
dc.language.isoenen_US
dc.publisherFrontiersen_US
dc.relation.ispartofseriesFrontiers in Microbiology;
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectmouseen_US
dc.subjectnisinen_US
dc.subjectstarchen_US
dc.subjectresistant starchen_US
dc.subjectmicrobiotaen_US
dc.subjectdigestionen_US
dc.subjectAkkermansiaen_US
dc.titleOral Delivery of Nisin in Resistant Starch Based Matrices Alters the Gut Microbiota in Miceen_US
dc.typeArticleen_US
dc.identifier.doihttps://dx.doi.org/10.3389/fmicb.2018.01186
dc.contributor.sponsorDepartment of Agriculture, Food and the Marineen_US
dc.contributor.sponsorTeagasc Walsh Fellowship Programmeen_US
dc.contributor.sponsorTeagascen_US
dc.contributor.sponsorScience Foundation Irelanden_US
dc.contributor.sponsorBiotechnology and Biological Sciences Research Councilen_US
dc.contributor.sponsorGrantNumber10/RD/TMFRC/701en_US
dc.contributor.sponsorGrantNumber2012221en_US
dc.contributor.sponsorGrantNumberSFI/12/RC2273en_US
dc.contributor.sponsorGrantNumberSFI/16/BBSRC/3389en_US
dc.contributor.sponsorGrantNumberBB/P009875/1en_US
refterms.dateFOA2019-08-13T11:00:52Z


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