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dc.contributor.authorO'Riordan, Noelle
dc.contributor.authorO'Callaghan, John
dc.contributor.authorButto, Ludovica F.
dc.contributor.authorKilcoyne, Michelle
dc.contributor.authorJoshi, Lokesh
dc.contributor.authorHickey, Rita M.
dc.date.accessioned2019-08-14T08:32:15Z
dc.date.available2019-08-14T08:32:15Z
dc.date.issued2018-05-24
dc.identifier.citationO'Riordan, N., O'Callaghan, J., Buttò, L., Kilcoyne, M., Joshi, L. and Hickey, R. (2018). Bovine glycomacropeptide promotes the growth of Bifidobacterium longum ssp. infantis and modulates its gene expression. Journal of Dairy Science, 101(8), 6730-6741. doi: https://dx.doi.org/10.3168/jds.2018-14499.en_US
dc.identifier.urihttp://hdl.handle.net/11019/1739
dc.descriptionpeer-revieweden_US
dc.description.abstractBovine milk glycomacropeptide (GMP) is derived from κ-casein, with exclusively o-linked glycosylation. Glycomacropeptide promoted the growth of Bifidobacterium longum ssp. infantis in a concentration-dependent manner, and this activity was lost following periodate treatment of the GMP (GMP-P), which disables biological recognition of the conjugated oligosaccharides. Transcriptional analysis of B. longum ssp. infantis following exposure to GMP revealed a substantial response to GMP relative to bacteria treated with GMP-P, with a greater number of differentially expressed transcripts and larger fold changes versus the control. Therefore, stimulation of B. longum ssp. infantis growth by GMP is intrinsically linked to the peptide's O-linked glycosylation. The pool of differentially expressed transcripts included 2 glycoside hydrolase (family 25) genes, which were substantially upregulated following exposure to GMP, but not GMP-P. These GH25 genes were present in duplicated genomic islands that also contained genes encoding fibronectin type III binding domain proteins and numerous phage-related proteins, all of which were also upregulated. Homologs of this genomic arrangement were present in other Bifidobacterium species, which suggest it may be a conserved domain for the utilization of glycosylated peptides. This study provides insights into the molecular basis for the prebiotic effect of bovine milk GMP on B. longum ssp. infantis.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofseriesJournal of Dairy Science;Vol. 101 (8)
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectbovine milken_US
dc.subjecttranscriptionen_US
dc.subjectglycomacropeptideen_US
dc.subjectBifidobacteriumen_US
dc.titleBovine glycomacropeptide promotes the growth of Bifidobacterium longum ssp. infantis and modulates its gene expressionen_US
dc.typeArticleen_US
dc.embargo.terms2019-05-24en_US
dc.identifier.doihttps://dx.doi.org/10.3168/jds.2018-14499
dc.contributor.sponsorTeagasc Walsh Fellowship Programmeen_US
refterms.dateFOA2019-05-24T00:00:00Z


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