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dc.contributor.authorGough, Ronan
dc.contributor.authorO'Connor, Paula M.
dc.contributor.authorRea, Mary
dc.contributor.authorGomez-Sala, Beatriz
dc.contributor.authorMiao, Song
dc.contributor.authorHill, Colin
dc.contributor.authorBrodkorb, Andre
dc.date.accessioned2019-08-14T13:58:14Z
dc.date.available2019-08-14T13:58:14Z
dc.date.issued2017-08-14
dc.identifier.citationGough, R., O'Connor, P., Rea, M., Gómez-Sala, B., Miao, S., Hill, C. and Brodkorb, A. (2017). Simulated gastrointestinal digestion of nisin and interaction between nisin and bile. LWT, 86, pp.530-537. Available at: https://dx.doi.org/10.1016/j.lwt.2017.08.031.en_US
dc.identifier.urihttp://hdl.handle.net/11019/1745
dc.descriptionpeer-revieweden_US
dc.description.abstractNisin, an antimicrobial peptide showing activity against many Gram positive bacteria, is widely used as a food preservative. The simulated gastrointestinal digestion of nisin (variant A) was studied using the in vitro INFOGEST digestion method. Following oral, gastric and small intestinal digestion, there was no intact nisin in the system and the nisin was primarily digested by pancreatin. After digestion, six nisin fragments (1–11, 1–12, 1–20, 1–21, 1–29 and 1–32) were identified by reversed phase high performance liquid chromatography and mass spectroscopy and four of these nisin fragments (1–20, 1–21, 1–29 and 1–32) demonstrated low antibacterial activity against Lactococcus lactis HP in agar diffusion activity assays. Additionally, it was observed that bile salts form a complex with nisin. This was examined by atomic force microscopy, turbidity and dynamic light scattering, which showed that this interaction resulted in significantly larger bile salt micelles. The presence of bile salts at physiological levels significantly altered the relative amounts of the nisin fragments 1–12, 1–20 and 1–29 produced during an in vitro digestion. This study highlights the importance of including bile in simulated digestions of antimicrobial peptides in order to obtain a more accurate simulation of the in vivo digestion products and their activity.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofseriesLWT - Food Science and Technology;Vol. 86
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectBileen_US
dc.subjectIn vitro digestionen_US
dc.subjectAntimicrobial peptideen_US
dc.subjectSurfactanten_US
dc.titleSimulated gastrointestinal digestion of nisin and interaction between nisin and bileen_US
dc.typeArticleen_US
dc.embargo.terms2018-08-14en_US
dc.identifier.doihttps://dx.doi.org/10.1016/j.lwt.2017.08.031
dc.contributor.sponsorDepartment of Agriculture, Food and the Marineen_US
dc.contributor.sponsorTeagasc Walsh Fellowship Programmeen_US
dc.contributor.sponsorGrantNumber10/RD/TMFRC/701en_US
refterms.dateFOA2018-08-14T00:00:00Z


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