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dc.contributor.authorMc Namara, Louise
dc.contributor.authorGriffin, Christine T.
dc.contributor.authorFitzpatrick, David
dc.contributor.authorKavanagh, Kevin
dc.contributor.authorCarolan, James C.
dc.date.accessioned2019-08-16T13:43:44Z
dc.date.available2019-08-16T13:43:44Z
dc.date.issued2018-07-17
dc.identifier.citationNamara, L., Griffin, C., Fitzpatrick, D., Kavanagh, K. and Carolan, J. (2018). The effect of entomopathogenic fungal culture filtrate on the immune response and haemolymph proteome of the large pine weevil, Hylobius abietis. Insect Biochemistry and Molecular Biology, 101, pp.1-13. doi: https://dx.doi.org/10.1016/j.ibmb.2018.07.001en_US
dc.identifier.urihttp://hdl.handle.net/11019/1754
dc.descriptionpeer-revieweden_US
dc.description.abstractThe large pine weevil Hylobius abietis L. is a major forestry pest in 15 European countries, where it is a threat to 3.4 million hectares of forest. A cellular and proteomic analysis of the effect of culture filtrate of three entomopathogenic fungi (EPF) species on the immune system of H. abietis was performed. Injection with Metarhizium brunneum or Beauvaria bassiana culture filtrate facilitated a significantly increased yeast cell proliferation in larvae. Larvae co-injected with either Beauvaria caledonica or B. bassiana culture filtrate and Candida albicans showed significantly increased mortality. Together these results suggest that EPF culture filtrate has the potential to modulate the insect immune system allowing a subsequent pathogen to proliferate. Injection with EPF culture filtrate was shown to alter the abundance of protease inhibitors, detoxifing enzymes, antimicrobial peptides and proteins involved in reception/detection and development in H. abietis larvae. Larvae injected with B. caledonica culture filtrate displayed significant alterations in abundance of proteins involved in cellulolytic and other metabolic processes in their haemolymph proteome. Screening EPF for their ability to modulate the insect immune response represents a means of assessing EPF for use as biocontrol agents, particularly if the goal is to use them in combination with other control agents.en_US
dc.description.sponsorshipThis research was funded by the Irish Government (Department of Agriculture, Food and the Marine) (10/RD/MCOP/NUIM/720) under the National Development Plan 2007–2013 and through the MU Department of Biology Contingency Fund. The Q-Exactive quantitative mass spectrometer was funded under the SFI Research Infrastructure Call 2012; Grant Number: 12/RI/2346 (3) to Prof. S. Doyle.
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofseriesInsect Biochemistry and Molecular Biology;Vol. 101
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectLawrge pine weevilen_US
dc.subjectEntomopathogenic fungien_US
dc.subjectProteomicen_US
dc.subjectTranscriptomeen_US
dc.subjectImmunomodulationen_US
dc.subjectBiocontrolen_US
dc.titleThe effect of entomopathogenic fungal culture filtrate on the immune response and haemolymph proteome of the large pine weevil, Hylobius abietisen_US
dc.typeArticleen_US
dc.embargo.terms2019-07-17en_US
dc.identifier.doihttps://dx.doi.org/10.1016/j.ibmb.2018.07.001
dc.contributor.sponsorDepartment of Agriculture, Food and the Marineen_US
dc.contributor.sponsorScience Foundation Irelanden_US
dc.contributor.sponsorGrantNumber10/RD/MCOP/NUIM/720en_US
dc.contributor.sponsorGrantNumber12/RI/2346 (3)en_US
refterms.dateFOA2019-07-17T00:00:00Z


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