Show simple item record

dc.contributor.authorMesa-Pereira, Beatriz
dc.contributor.authorO’Connor, Paula M.
dc.contributor.authorRea, Mary C.
dc.contributor.authorCotter, Paul D.
dc.contributor.authorHill, Colin
dc.contributor.authorRoss, R Paul
dc.date.accessioned2020-02-13T16:15:01Z
dc.date.available2020-02-13T16:15:01Z
dc.date.issued2017-06-08
dc.identifier.citationMesa-Pereira, B., O’Connor, P.M., Rea, M.C. et al. Controlled functional expression of the bacteriocins pediocin PA-1 and bactofencin A in Escherichia coli . Sci Rep 7, 3069 (2017). https://doi.org/10.1038/s41598-017-02868-wen_US
dc.identifier.urihttp://hdl.handle.net/11019/1865
dc.descriptionpeer-revieweden_US
dc.description.abstractThe bacteriocins bactofencin A (class IId) and pediocin PA-1 (class IIa) are encoded by operons with a similarly clustered gene organization including a structural peptide, an immunity protein, an ABC transporter and accessory bacteriocin transporter protein. Cloning of these operons in E. coli TunerTM (DE3) on a pETcoco-2 derived vector resulted in successful secretion of both bacteriocins. A corresponding approach, involving the construction of vectors containing different combinations of these genes, revealed that the structural and the transporter genes alone are sufficient to permit heterologous production and secretion in this host. Even though the accessory protein, usually associated with optimal disulfide bond formation, was not required for bacteriocin synthesis, its presence did result in greater pediocin PA-1 production. The simplicity of the system and the fact that the associated bacteriocins could be recovered from the extracellular medium provides an opportunity to facilitate protein engineering and the overproduction of biologically-active bacteriocins at industrial scale. Additionally, this system could enable the characterization of new bacteriocin operons where genetic tools are not available for the native producers.en_US
dc.language.isoenen_US
dc.publisherNature Publishing Groupen_US
dc.relation.ispartofseriesScientific Reports;Vol. 7 (1)
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectbactofencin Aen_US
dc.subjectEscherichia colien_US
dc.subjectbacteriocinen_US
dc.subjectpediocinen_US
dc.subjectbacteriaen_US
dc.subjectntiinfective agentsen_US
dc.titleControlled functional expression of the bacteriocins pediocin PA-1 and bactofencin A in Escherichia colien_US
dc.typeArticleen_US
dc.identifier.doihttps://dx.doi.org/10.1038/s41598-017-02868-w
dc.contributor.sponsorScience Foundation Irelanden_US
dc.contributor.sponsorGrantNumberSFI/12/RC/2273en_US
refterms.dateFOA2020-02-13T16:15:02Z


Files in this item

Thumbnail
Name:
s41598-017-02868-w.pdf
Size:
1.995Mb
Format:
PDF
Description:
main article

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-ShareAlike 3.0 United States
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 3.0 United States