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dc.contributor.authorRyan, Paul M
dc.contributor.authorPatterson, Elaine
dc.contributor.authorKent, Robert M.
dc.contributor.authorStack, Helena
dc.contributor.authorO’Connor, Paula M.
dc.contributor.authorMurphy, Kiera
dc.contributor.authorPeterson, Veronica L.
dc.contributor.authorMandal, Rupasri
dc.contributor.authorWishart, David S.
dc.contributor.authorDinan, Timothy G.
dc.contributor.authorCryan, John F.
dc.contributor.authorSeeley, Randy J.
dc.contributor.authorSTANTON, CATHERINE
dc.contributor.authorRoss, R Paul
dc.date.accessioned2020-03-30T12:07:53Z
dc.date.available2020-03-30T12:07:53Z
dc.date.issued2017-10-19
dc.identifier.citationRyan, P., Patterson, E., Kent, R., Stack, H., O’Connor, P., Murphy, K., Peterson, V., Mandal, R., Wishart, D., Dinan, T., Cryan, J., Seeley, R., Stanton, C. and Ross, R. Recombinant Incretin-Secreting Microbe Improves Metabolic Dysfunction in High-Fat Diet Fed Rodents. Scientific Reports, 2017, 7(1), 13523. doi: https://dx.doi.org/10.1038/s41598-017-14010-xen_US
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11019/1880
dc.descriptionpeer-revieweden_US
dc.description.abstractThe gut hormone glucagon-like peptide (GLP)-1 and its analogues represent a new generation of anti-diabetic drugs, which have also demonstrated propensity to modulate host lipid metabolism. Despite this, drugs of this nature are currently limited to intramuscular administration routes due to intestinal degradation. The aim of this study was to design a recombinant microbial delivery vector for a GLP-1 analogue and assess the efficacy of the therapeutic in improving host glucose, lipid and cholesterol metabolism in diet induced obese rodents. Diet-induced obese animals received either Lactobacillus paracasei NFBC 338 transformed to express a long-acting analogue of GLP-1 or the isogenic control microbe which solely harbored the pNZ44 plasmid. Short-term GLP-1 microbe intervention in rats reduced serum low-density lipoprotein cholesterol, triglycerides and triglyceride-rich lipoprotein cholesterol substantially. Conversely, extended GLP-1 microbe intervention improved glucose-dependent insulin secretion, glucose metabolism and cholesterol metabolism, compared to the high-fat control group. Interestingly, the microbe significantly attenuated the adiposity associated with the model and altered the serum lipidome, independently of GLP-1 secretion. These data indicate that recombinant incretin-secreting microbes may offer a novel and safe means of managing cholesterol metabolism and diet induced dyslipidaemia, as well as insulin sensitivity in metabolic dysfunction.en_US
dc.language.isoenen_US
dc.publisherSpringer Natureen_US
dc.relation.ispartofseriesScientific Reports;Vol. 7(1)
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectMetabolic Dysfunctionen_US
dc.subjectHigh-Fat Dietsen_US
dc.subject(GLP)-1en_US
dc.subjectincretin-secreting microbesen_US
dc.titleRecombinant Incretin-Secreting Microbe Improves Metabolic Dysfunction in High-Fat Diet Fed Rodentsen_US
dc.typeArticleen_US
dc.identifier.doihttps://dx.doi.org/10.1038/s41598-017-14010-x
dc.contributor.sponsorScience Foundation Irelanden_US
dc.contributor.sponsorTeagasc Walsh Fellowship Programmeen_US
dc.contributor.sponsorGrantNumberSFI/12/RC/2273en_US
dc.source.volume7
dc.source.issue1
refterms.dateFOA2020-03-30T12:07:54Z


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