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dc.contributor.authorMcGovern, Emily
dc.contributor.authorMcCabe, Matthew
dc.contributor.authorCormican, Paul
dc.contributor.authorPopova, Milka
dc.contributor.authorKeogh, Kate
dc.contributor.authorKelly, Alan K
dc.contributor.authorKenny, David A.
dc.contributor.authorWaters, Sinead M.
dc.date.accessioned2020-03-30T12:16:04Z
dc.date.available2020-03-30T12:16:04Z
dc.date.issued2017-10-12
dc.identifier.citationMcGovern, E., McCabe, M., Cormican, P., Popova, M., Keogh, K., Kelly, A., Kenny, D. and Waters, S. (2017). Plane of nutrition affects the phylogenetic diversity and relative abundance of transcriptionally active methanogens in the bovine rumen. Scientific Reports, 2017, 7(1). doi: https://dx.doi.org/10.1038/s41598-017-13013-yen_US
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11019/1881
dc.descriptionpeer-revieweden_US
dc.description.abstractMethane generated during enteric fermentation in ruminant livestock species is a major contributor to global anthropogenic greenhouse gas emissions. A period of moderate feed restriction followed by ad libitum access to feed is widely applied in cattle management to exploit the animal’s compensatory growth potential and reduce feed costs. In the present study, we utilised microbial RNA from rumen digesta samples to assess the phylogenetic diversity of transcriptionally active methanogens from feed-restricted and non-restricted animals. To determine the contribution of different rumen methanogens to methanogenesis during dietary restriction of cattle, we conducted high-throughput mcrA cDNA amplicon sequencing on an Illumina MiSeq and analysed both the abundance and phylogenetic origin of different mcrA cDNA sequences. When compared to their unrestricted contemporaries, in feed-restricted animals, the methanogenic activity, based on mcrA transcript abundance, of Methanobrevibacter gottschalkii clade increased while the methanogenic activity of the Methanobrevibacter ruminantium clade and members of the Methanomassiliicoccaceae family decreased. This study shows that the quantity of feed consumed can evoke large effects on the composition of methanogenically active species in the rumen of cattle. These data potentially have major implications for targeted CH4 mitigation approaches such as anti-methanogen vaccines and/or tailored dietary management.en_US
dc.language.isoenen_US
dc.publisherSpringer Natureen_US
dc.relation.ispartofseriesScientific Reports;Vol. 7 (1)
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectbovine rumenen_US
dc.subjectMethaneen_US
dc.subjectruminant livestocken_US
dc.subjectcattle managementen_US
dc.subjectanthropogenic greenhouse gas emissions
dc.subjectrumen methanogens
dc.titlePlane of nutrition affects the phylogenetic diversity and relative abundance of transcriptionally active methanogens in the bovine rumenen_US
dc.typeArticleen_US
dc.identifier.doihttps://dx.doi.org/10.1038/s41598-017-13013-y
dc.source.volume7
dc.source.issue1
refterms.dateFOA2020-03-30T12:16:05Z


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