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dc.contributor.authorAjuebor, Jude
dc.contributor.authorButtimer, Colin
dc.contributor.authorArroyo-Moreno, Sara
dc.contributor.authorChanishvili, Nina
dc.contributor.authorGabriel, Emma
dc.contributor.authorO’Mahony, Jim
dc.contributor.authorMcAuliffe, Olivia
dc.contributor.authorNeve, Horst
dc.contributor.authorFranz, Charles
dc.contributor.authorCoffey, Aidan
dc.date.accessioned2020-06-03T13:41:46Z
dc.date.available2020-06-03T13:41:46Z
dc.date.issued2018-04-25
dc.identifier.citationAjuebor, J., Buttimer, C., Arroyo-Moreno, S., Chanishvili, N., Gabriel, E., O’Mahony, J., McAuliffe, O., Neve, H., Franz, C. and Coffey, A. Comparison of Staphylococcus Phage K with Close Phage Relatives Commonly Employed in Phage Therapeutics. Antibiotics, 2018, 7(2), 37. Available at: https://dx.doi.org/10.3390/antibiotics7020037en_US
dc.identifier.issn2079-6382
dc.identifier.urihttp://hdl.handle.net/11019/1910
dc.descriptionpeer-revieweden_US
dc.description.abstractThe increase in antibiotic resistance in pathogenic bacteria is a public health danger requiring alternative treatment options, and this has led to renewed interest in phage therapy. In this respect, we describe the distinct host ranges of Staphylococcus phage K, and two other K-like phages against 23 isolates, including 21 methicillin-resistant S. aureus (MRSA) representative sequence types representing the Irish National MRSA Reference Laboratory collection. The two K-like phages were isolated from the Fersisi therapeutic phage mix from the Tbilisi Eliava Institute, and were designated B1 (vB_SauM_B1) and JA1 (vB_SauM_JA1). The sequence relatedness of B1 and JA1 to phage K was observed to be 95% and 94% respectively. In terms of host range on the 23 Staphylococcus isolates, B1 and JA1 infected 73.9% and 78.2% respectively, whereas K infected only 43.5%. Eleven open reading frames (ORFs) present in both phages B1 and JA1 but absent in phage K were identified by comparative genomic analysis. These ORFs were also found to be present in the genomes of phages (Team 1, vB_SauM-fRuSau02, Sb_1 and ISP) that are components of several commercial phage mixtures with reported wide host ranges. This is the first comparative study of therapeutic staphylococcal phages within the recently described genus Kayvirus.en_US
dc.language.isoenen_US
dc.publisherMDPI AGen_US
dc.relation.ispartofseriesAntibiotics;Vol. 7 (2)
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectphage isolationen_US
dc.subjectbacteriophageen_US
dc.subjectphage resistanceen_US
dc.subjectMRSAen_US
dc.subjectStaphylococcusen_US
dc.subjectKayvirusen_US
dc.titleComparison of Staphylococcus Phage K with Close Phage Relatives Commonly Employed in Phage Therapeuticsen_US
dc.typeArticleen_US
dc.identifier.doihttps://dx.doi.org/10.3390/antibiotics7020037
dc.contributor.sponsorScience Foundation Irelanden_US
dc.contributor.sponsorGrantNumber12/R1/2335en_US
dc.source.volume7
dc.source.issue2
dc.source.beginpage37
refterms.dateFOA2020-06-03T13:41:47Z


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