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dc.contributor.authorSuda, Srinivas
dc.contributor.authorWesterbeek, Alja
dc.contributor.authorO'Connor, Paula M.
dc.contributor.authorHill, Colin
dc.contributor.authorCotter, Paul D.
dc.contributor.authorRoss, R Paul
dc.date.accessioned2020-06-09T16:13:23Z
dc.date.available2020-06-09T16:13:23Z
dc.date.issued2010-10-28
dc.identifier.citationSuda, S., Westerbeek, A., O'Connor, P., Ross, R., Hill, C. and Cotter, P. Effect of Bioengineering Lacticin 3147 Lanthionine Bridges on Specific Activity and Resistance to Heat and Proteases. Chemistry & Biology, 2010, 17(10), 1151-1160. doi: https://dx.doi.org/10.1016/j.chembiol.2010.08.011en_US
dc.identifier.issn1074-5521
dc.identifier.urihttp://hdl.handle.net/11019/1957
dc.descriptionpeer-revieweden_US
dc.description.abstractLacticin 3147 is a lantibiotic with seven lanthionine bridges across its two component peptides, Ltnα and Ltnβ. Although it has been proposed that the eponymous lanthionine and (β-methyl)lanthionine (Lan and meLan) bridges present in lantibiotics make an important contribution to protecting the peptides from thermal or proteolytic degradation, few studies have investigated this link. We have generated a bank of bioengineered derivatives of lacticin 3147, in which selected bridges were removed or converted between Lan and meLan, which were exposed to high temperature or proteolytic enzymes. Although switching Lan and meLan bridges has variable consequences, it was consistently observed that an intact N-terminal lanthionine bridge (Ring A) confers Ltnα with enhanced resistance to thermal and proteolytic degradation.en_US
dc.language.isoenen_US
dc.publisherElsevier BVen_US
dc.relation.ispartofseriesChemistry & Biology;Vol. 17 (10)
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectClinical Biochemistryen_US
dc.subjectMolecular Medicineen_US
dc.subjectBiochemistryen_US
dc.subjectMolecular Biologyen_US
dc.subjectPharmacologyen_US
dc.subjectDrug Discoveryen_US
dc.subjectGeneral Medicineen_US
dc.titleEffect of Bioengineering Lacticin 3147 Lanthionine Bridges on Specific Activity and Resistance to Heat and Proteasesen_US
dc.typeArticleen_US
dc.identifier.doihttps://dx.doi.org/10.1016/j.chembiol.2010.08.011
dc.contributor.sponsorScience Foundation Irelanden_US
dc.contributor.sponsorGrantNumber06/IN.1/B98en_US
dc.source.volume17
dc.source.issue10
dc.source.beginpage1151-1160
refterms.dateFOA2020-06-09T16:13:23Z


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