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dc.contributor.authorLi, Fang
dc.contributor.authorHunt, Karen
dc.contributor.authorVan Hoorde, Koenraad
dc.contributor.authorButler, Francis
dc.contributor.authorJordan, Kieran
dc.contributor.authorTobin, John
dc.creatorFang, Li
dc.date.accessioned2020-06-16T16:05:55Z
dc.date.available2020-06-16T16:05:55Z
dc.date.issued2019-05-28
dc.identifier.citationLi, F., Hunt, K., Van Hoorde, K., Butler, F., Jordan, K. and Tobin, J. Occurrence and identification of spore-forming bacteria in skim-milk powders. International Dairy Journal, 2019, 97, 176-184. doi: https://doi.org/10.1016/j.idairyj.2019.05.004en_US
dc.identifier.issn09586946
dc.identifier.urihttp://hdl.handle.net/11019/1994
dc.descriptionpeer-revieweden_US
dc.description.abstractThe different customer and regulatory specifications for mesophilic and thermophilic aerobic and anaerobic spore numbers in skim-milk powder, in addition to some specifications on specific spore-forming bacteria, such as Bacillus cereus, can be challenging for the industry to meet. Twenty-two samples of medium-heat skim-milk spray-dried powder from eight sources were analysed in triplicate with 16 bacterial and spore enumeration tests to understand the variety of spore-forming bacteria population. Using 16S rDNA sequencing, the species were identified for 269 isolates that were representative of the various tests. Of the isolates identified, 68% were Bacillus licheniformis, a facultative anaerobe that can survive and grow at mesophilic and thermophilic temperatures, making it difficult to eliminate in manufacturing environments. Using whole genome sequencing, 16 of 23 isolates identified as B. licheniformis by 16S sequencing were confirmed as B. licheniformis, four were identified as Bacillus paralicheniformis and three were identified as Bacillus sp. H15-1.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofInternational Dairy Journal
dc.relation.ispartofseriesInternational Dairy Journal;Vol. 97
dc.rights© 2019 Elsevier Ltd. All rights reserved.
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectspore-forming bacteriaen_US
dc.subjectskim-milk powdersen_US
dc.subject16S rDNA sequencingen_US
dc.subjectBacillus licheniformisen_US
dc.subjectBacillus cereusen_US
dc.subjectBacillus paralicheniformisen_US
dc.subjectBacillus sp. H15-1en_US
dc.titleOccurrence and identification of spore-forming bacteria in skim-milk powdersen_US
dc.typeArticleen_US
dc.embargo.terms2020-05-28en_US
dc.identifier.doihttps://doi.org/10.1016/j.idairyj.2019.05.004
dc.relation.volume97
dc.contributor.sponsorDepartment of Agriculture, Food and the Marineen_US
dc.contributor.sponsorEnterprise Irelanden_US
dc.contributor.sponsorGrantNumber14/F/883en_US
dc.contributor.sponsorGrantNumberTC 2014 0016en_US
dc.source.volume97
dc.source.beginpage176
dc.source.endpage184
refterms.dateFOA2020-05-28T00:00:00Z


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© 2019 Elsevier Ltd. All rights reserved.
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