Browsing Food Programme by Author "Adhikari, Benu"
Effects of transglutaminase pre-crosslinking on salt-induced gelation of soy protein isolate emulsionLuo, Kaiyun; Liu, Shutao; Miao, Song; Adhikari, Benu; Wang, Xufeng; Chen, Jie; National Natural Science Foundation of China; National High-Tech Research and Development Program of China; National Natural Science Foundation of China; NSFC, 31271946; et al. (Elsevier, 2019-07-09)The salt-induced gelation behavior of soy protein isolate (SPI) emulsions was markedly influenced by microbial transglutaminase (TGase) pre-crosslinking. Rheological data showed that when SPI emulsions were incubated with TGase at low concentrations (1 and 3 U/g protein) at 50 °C for 30 min prior to gelation, no change in storage modulus (G′), but enhanced resistance to deformation of the gels was observed. Extensive crosslinking by TGase (5 U/g protein) resulted in severe decreases in gel firmness and fracture properties (yielding stress and strain), likely due to the impairment of hydrophobic bonds and the formation of coarse networks. The water-holding capacity of the gels was significantly enhanced by increased concentrations of TGase. Interactive force analysis indicated that non-covalent interactions and disulfide bonds are the primary forces involved in CaSO4-induced SPI emulsion gel, but TGase treatment may limit hydrophobic interactions within the gel network. These results are of great potential value for the application of TGase in the food industry.