• Effects of oligosaccharides on particle structure, pasting and thermal properties of wheat starch granules under different freezing temperatures

      Su, Han; Tu, Jinjin; Zheng, Mingging; Deng, Kaibo; Miao, Song; Zeng, Shaoxiao; Zheng, Baodong; Lu, Xu; National Natural Science Foundation of China; Science and Technology Major Project of Fujian Province of China; et al. (Elsevier, 2020-06-15)
      The effects of fructooligosaccharides (FOS), galactooligosaccharides (GOS), and xylooligosaccharides (XOS) on gelatinization, retrogradation, thermal properties and particle size of wheat starch at different freezing temperatures were studied. The results showed that the wheat starch porosity, particle size, peak viscosity increased with increasing freezing temperature. With the addition of 16% oligosaccharides to starch, the porosity, particle size, crystallinity, initial gelatinization temperature, peak value, breakdown and retrogradation viscosity of the starch granules significantly decreased in the order of XOS > GOS > FOS. However, the pasting temperature of the granules increased. The addition of oligosaccharides (especially XOS, which has the most significant effect in inhibiting starch retrogradation) can inhibit the formation of starch crystal structures to a certain extent, reduce the damage from ice crystals to starch granules and delay starch retrogradation. Therefore, functional oligosaccharides can be used as a potentially effective additive to increase freezing stability in frozen starch-based foods.
    • Lotus seed oligosaccharides at various dosages with prebiotic activity regulate gut microbiota and relieve constipation in mice.

      Su, Han; Chen, Jinghao; Miao, Song; Deng, Kaibo; Liu, Jiawen; Zeng, Shaoxiao; Zheng, Baodong; Lu, Xu; China-Ireland International Cooperation Centre; National Natural Science Foundation of China; et al. (Elsevier, 2019-09-27)
      The aim of this study was to evaluate the effects of lotus seed oligosaccharides (formulation consisting of LSO2, LSO3-1, LSO3-2 and LSO4; relative ratios are 1.107:0.554: 0.183:0.443, m/m/m/m) at dosages of 0.42, 0.83 g/d/kg bw and 2.49 g/d/kg bw on the microbiota composition and the propulsion of intestinal contents in the gut of mice. The results showed that fecal water content increased in treated mice; there was less gut microbiota diversity than in other groups; and there was a large number of fauna in the cecum of the mice. At the same time, the number of short-chain fatty acid (SCFA) bacterial producers increased after feeding with oligosaccharides; Lotus seed oligosaccharides (LOS) also enhanced the concentration of SCFAs in the intestine, which also increased the concentration of cytokines in the serum of mice. In conclusion, these findings suggest that LOS or combination with resistant starch has a better effect on relieving constipation.
    • Paste structure and rheological properties of lotus seed starch–glycerin monostearate complexes formed by high-pressure homogenization

      Chen, Bingyan; Guo, Zebin; Zeng, Shaoxiao; Tian, Yuting; Miao, Song; Zheng, Baodong; National Natural Science Foundation of China; Scientific and Technological Innovation Team Support Plan of Institution of Higher Learning in Fujian Province; Fujian Agriculture and Forestry University of China; 31501485; et al. (Elsevier, 2017-10-31)
      Starch–lipid complexes were prepared using lotus seed starch (LS) and glycerin monostearate (GMS) via a high-pressure homogenization (HPH) process, and the effect of HPH on the paste structure and rheological properties of LS–GMS was investigated. Rapid Visco Analyser (RVA) profiles showed that HPH treatment inhibited the formation of the second viscosity peak of the LS–GMS paste, and the extent of this change was dependent on the level of homogenized pressure. Analysis of the size-exclusion chromatography, light microscopy, and low-field 1H nuclear magnetic resonance results revealed that high homogenized pressure (70–100 MPa) decreased molecular weight and size by degrading the branch structure of amylopectin; however, intact LS–GMS granules can optimize the network structure by filler–matrix interaction, which causes free water to transition into immobile water in the starch paste. The steady-shear results showed that the LS–GMS pastes presented non-Newtonian shear-thinning behavior, with higher homogenized pressure producing a smaller hysteresis loop area. During the oscillation process, the LS–GMS pastes prepared at 100 MPa exhibited the lowest loss tangent values in all the complexes, indicating a stronger resistance to vibration.
    • Preparation and characterization of lotus seed starch-fatty acid complexes formed by microfluidization

      Chen, Bingyan; Guo, Zebin; Miao, Song; Zeng, Shaoxiao; Jia, Xiangze; Zhang, Yi; Zheng, Baodong; National Natural Science Foundation of China; Scientific and Technological Innovation Team Support Plan of Institution of Higher Learning in Fujian Province; Construction Projects of Top University; et al. (Elsevier, 2018-05-22)
      Using dynamic high pressure microfluidization, we prepared starch-lipid complexes from lotus seed starch (LS) and six saturated fatty acids (FAs) of different carbon chain length and analyzed their semi-crystalline structure and digestibility. Iodine blue value analysis showed the highest complex index (86.3%) was observed between LS and octanoic acid (C8). X-ray diffraction analysis showed crystal structure changed from V6II to V6I type with decreasing FA chain length. Small angle x-ray scattering and differential scanning calorimetry analyses confirmed the presence of a strong V6I-type mass fractal structure with a Bragg distance of 12.3 nm in LS-C8, which can be considered to be a type-II complex with high melting temperature (Tp = 123.98 °C). Scanning electron microscopy results showed the complexes had more spherocrystals with decreasing FA chain length. Compared to other FAs, C8 significantly reduced the LS susceptibility to digestive enzymes, increased slowly digestion starch content (26.06%) and decreased digestion rate (3.59 × 10−2).
    • Rheological properties and structural features of coconut milk emulsions stabilized with maize kernels and starch

      Lu, Xu; Su, Han; Guo, Juanjuan; Tu, Jinjin; Lei, Yi; Zeng, Shaoxiao; Chen, Yingtong; Miao, Song; Zheng, Baodong; China-Ireland International Cooperation Centre for Food Material Science and Structure Design; et al. (Elsevier, 2019-05-16)
      In this study, maize kernels and starch with different amylose contents at the same concentration were added to coconut milk. The nonionic composite surfactants were used to prepare various types of coconut milk beverages with optimal stability, and their fluid properties were studied. The steady and dynamic rheological property tests show that the loss modulus (G″) of coconut milk is larger than the storage modulus (G′), which is suitable for the pseudoplastic fluid model and has a shear thinning effect. As the droplet size of the coconut milk fluid changed by the addition of maize kernels and starch, the color intensity, ζ-potential, interfacial tension and stability of the sample significantly improved. The addition of the maize kernels significantly reduced the size of the droplets (p < 0.05). The potential values of zeta (ζ) and the surface tension of the coconut milk increased. Based on the differential scanning calorimetry (DSC) measurement, the addition of maize kernels leads to an increase in the transition temperature, especially in samples with a high amylose content. The higher transition temperature can be attributed to the formation of some starches and lipids and the partial denaturation of proteins in coconut milk, but phase separation occurs. These results may be helpful for determining the properties of maize kernels in food-containing emulsions (such as sauces, condiments, and beverages) that achieve the goal of physical stability.
    • Slowly digestible properties of lotus seed starch-glycerine monostearin complexes formed by high pressure homogenization

      Chen, Bingyan; Jia, Xiangze; Miao, Song; Zeng, Shaoxiao; Guo, Zebin; Zhang, Yi; Zheng, Baodong; National Natural Science Foundation of China; Scientific and Technological Innovation Team Support Plan of Institution of Higher Learning in Fujian Province; Fujian Agriculture and Forestry university doctoral programme; et al. (Elsevier, 2018-01-06)
      Starch-lipid complexes were prepared using lotus seed starch (LS) and glycerin monostearate (GMS) via a high-pressure homogenization process, and the effect of high pressure homogenization (HPH) on the slow digestion properties of LS-GMS was investigated. The digestion profiles showed HPH treatment reduced the digestive rate of LS-GMS, and the extent of this change was dependent on homogenized pressure. Scanning electron microscopy displayed HPH treatment change the morphology of LS-GMS, with high pressure producing more compact block-shape structure to resist enzyme digestion. The results of Gel-permeation chromatography and Small-angle X-ray scattering revealed high homogenization pressure impacted molecular weight distribution and semi-crystalline region of complexes, resulting in the formation of new semi-crystalline with repeat unit distance of 16–18 nm and molecular weight distribution of 2.50–2.80 × 105 Da, which displayed strong enzymatic resistance. Differential scanning calorimeter results revealed new semi-crystalline lamellar may originate from type-II complexes that exhibited a high transition temperature.