• Effect of ultrasound on physicochemical properties of emulsion stabilized by fish myofibrillar protein and xanthan gum

      Xiong, Yao; Li, Qianru; Miao, Song; Zhang, Yi; Zheng, Baodong; Zhang, Longtao; Fujian Agriculture and Forestry University; Fujian Provincial Foreign Cooperation Project; Fujian Provincial Science and Technology Program of Regional Development Project; National Natural Science Foundation of China; et al. (Elsevier, 2019-04-30)
      To investigate the effects ultrasound (20 kHz, 150–600 W) on physicochemical properties of emulsion stabilized by myofibrillar protein (MP) and xanthan gum (XG), the emulsions were characterized by Fourier transform infrared (FT-IR) spectroscopy, ζ-potential, particle size, rheology, surface tension, and confocal laser scanning microscopy (CLSM). FT-IR spectra confirmed the complexation of MP and XG, and ultrasound did not change the functional groups in the complexes. The emulsion treated at 300 W showed the best stability, with the lowest particle size, the lowest surface tension (26.7 mNm−1) and the largest ζ-potential absolute value (25.4 mV), that were confirmed in the CLSM photos. Ultrasound reduced the apparent viscosity of the MP-XG emulsions, and the changes of particle size were manifested in flow properties. Generally, ultrasound was successfully applied to improve the physical stability of MP-XG emulsion, which could be used as a novel delivery system for functional material.
    • Preparation and characterization of lotus seed starch-fatty acid complexes formed by microfluidization

      Chen, Bingyan; Guo, Zebin; Miao, Song; Zeng, Shaoxiao; Jia, Xiangze; Zhang, Yi; Zheng, Baodong; National Natural Science Foundation of China; Scientific and Technological Innovation Team Support Plan of Institution of Higher Learning in Fujian Province; Construction Projects of Top University; et al. (Elsevier, 2018-05-22)
      Using dynamic high pressure microfluidization, we prepared starch-lipid complexes from lotus seed starch (LS) and six saturated fatty acids (FAs) of different carbon chain length and analyzed their semi-crystalline structure and digestibility. Iodine blue value analysis showed the highest complex index (86.3%) was observed between LS and octanoic acid (C8). X-ray diffraction analysis showed crystal structure changed from V6II to V6I type with decreasing FA chain length. Small angle x-ray scattering and differential scanning calorimetry analyses confirmed the presence of a strong V6I-type mass fractal structure with a Bragg distance of 12.3 nm in LS-C8, which can be considered to be a type-II complex with high melting temperature (Tp = 123.98 °C). Scanning electron microscopy results showed the complexes had more spherocrystals with decreasing FA chain length. Compared to other FAs, C8 significantly reduced the LS susceptibility to digestive enzymes, increased slowly digestion starch content (26.06%) and decreased digestion rate (3.59 × 10−2).
    • Separation of Oligosaccharides from Lotus Seeds via Medium-pressure Liquid Chromatography Coupled with ELSD and DAD

      Lu, Xu; Zheng, Zhichang; Miao, Song; Li, Huang; Guo, Zebin; Zhang, Yi; Zheng, Yafeng; Zheng, Baodong; Xiao, Jianbo; National Natural Science Foundation of China; et al. (Springer Science, 2017-03-09)
      Lotus seeds were identified by the Ministry of Public Health of China as both food and medicine. One general function of lotus seeds is to improve intestinal health. However, to date, studies evaluating the relationship between bioactive compounds in lotus seeds and the physiological activity of the intestine are limited. In the present study, by using medium pressure liquid chromatography coupled with evaporative light-scattering detector and diode-array detector, five oligosaccharides were isolated and their structures were further characterized by electrospray ionization-mass spectrometry and gas chromatography-mass spectrometry. In vitro testing determined that LOS3-1 and LOS4 elicited relatively good proliferative effects on Lactobacillus delbrueckii subsp. bulgaricus. These results indicated a structure-function relationship between the physiological activity of oligosaccharides in lotus seeds and the number of probiotics applied, thus providing room for improvement of this particular feature. Intestinal probiotics may potentially become a new effective drug target for the regulation of immunity.
    • Slowly digestible properties of lotus seed starch-glycerine monostearin complexes formed by high pressure homogenization

      Chen, Bingyan; Jia, Xiangze; Miao, Song; Zeng, Shaoxiao; Guo, Zebin; Zhang, Yi; Zheng, Baodong; National Natural Science Foundation of China; Scientific and Technological Innovation Team Support Plan of Institution of Higher Learning in Fujian Province; Fujian Agriculture and Forestry university doctoral programme; et al. (Elsevier, 2018-01-06)
      Starch-lipid complexes were prepared using lotus seed starch (LS) and glycerin monostearate (GMS) via a high-pressure homogenization process, and the effect of high pressure homogenization (HPH) on the slow digestion properties of LS-GMS was investigated. The digestion profiles showed HPH treatment reduced the digestive rate of LS-GMS, and the extent of this change was dependent on homogenized pressure. Scanning electron microscopy displayed HPH treatment change the morphology of LS-GMS, with high pressure producing more compact block-shape structure to resist enzyme digestion. The results of Gel-permeation chromatography and Small-angle X-ray scattering revealed high homogenization pressure impacted molecular weight distribution and semi-crystalline region of complexes, resulting in the formation of new semi-crystalline with repeat unit distance of 16–18 nm and molecular weight distribution of 2.50–2.80 × 105 Da, which displayed strong enzymatic resistance. Differential scanning calorimeter results revealed new semi-crystalline lamellar may originate from type-II complexes that exhibited a high transition temperature.