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dc.contributor.authorFox, Aaron
dc.contributor.authorIkoyi, Israel
dc.contributor.authorTorres-Sallan, Gemma
dc.contributor.authorLanigan, Gary
dc.contributor.authorSchmalenberger, Achim
dc.contributor.authorWakelin, Steve
dc.contributor.authorCreamer, Rachel
dc.date.accessioned2020-06-23T12:00:01Z
dc.date.available2020-06-23T12:00:01Z
dc.date.issued2018-03-09
dc.identifier.citationFox, A., Ikoyi, I., Torres-Sallan, G., Lanigan, G., Schmalenberger, A., Wakelin, S. and Creamer, R. The influence of aggregate size fraction and horizon position on microbial community composition. Applied Soil Ecology, 2018, 127, 19-29. doi: https://dx.doi.org/10.1016/j.apsoil.2018.02.023en_US
dc.identifier.issn0929-1393
dc.identifier.urihttp://hdl.handle.net/11019/2047
dc.descriptionpeer-revieweden_US
dc.description.abstractThe influence of horizon position and aggregate size on bacterial and fungal community composition was determined. From nine sites, soils were collected from the top three horizon positions (H1, H2 and H3). Physical fractionation separated samples into large macroaggregate (LM, >2000 μm), macroaggregate (MAC, >250 μm), microaggregate (MIC, <250 μm), and silt and clay (SC, 53 μm) fractions. In all samples, the structure of the bacterial and fungal community composition was assessed via restriction fragment length polymorphism (T-RFLP), and for the four aggregate sizes from the top two horizons positions an in-depth analysis of the bacterial community was conducted using next generation sequencing (NGS). Bacterial and fungal communities both differed between aggregate-sizes. Changes in the composition of the bacterial and fungal communities also occurred among horizon positions, with a significant interaction between aggregate size and horizon position evident for the bacterial community. Using NGS, it was shown that aggregate-size had a significant effect on the bacterial community in both horizon positions at both the phyla and family taxonomic levels. MAC and MIC significantly differed in the % relative abundance of bacterial groups, potentially indicating differing predation pressures. These results indicate that both horizon position and aggregate size support distinct microbial communities. Understanding these parameters is critical in our comprehension of the patterns of microbial diversity in soil.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofseriesApplied Soil Ecology;Vol. 127
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectBacteriaen_US
dc.subjectFungien_US
dc.subjectAggregate sized fractionen_US
dc.subjectHorizon positionen_US
dc.subjectCommunity profilingen_US
dc.subjectNext generation sequencingen_US
dc.titleThe influence of aggregate size fraction and horizon position on microbial community compositionen_US
dc.typeArticleen_US
dc.embargo.terms2020-03-09en_US
dc.identifier.doihttps://dx.doi.org/10.1016/j.apsoil.2018.02.023
dc.contributor.sponsorTeagasc Walsh Fellowship Programmeen_US
dc.contributor.sponsorEnvironmental Protection Agencyen_US
dc.contributor.sponsorAgricultural GHG Research Initiative for Irelanden_US
dc.contributor.sponsorGrantNumber2013017en_US
dc.contributor.sponsorGrantNumber2007-SCD-1-S1en_US
dc.contributor.sponsorGrantNumber10/RD/AGRI-I/716en_US
refterms.dateFOA2020-03-09T00:00:00Z


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