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dc.contributor.authorMadden, Robert H
dc.contributor.authorHutchison, Mike
dc.contributor.authorJordan, Kieran
dc.contributor.authorPennone, Vincenzo
dc.contributor.authorGundogdu, Ozan
dc.contributor.authorCorcionivoschi, Nicolae
dc.identifier.citationMadden, R., Hutchison, M., Jordan, K., Pennone, V., Gundogdu, O. and Corcionivoschi, N. Prevalence and persistence of Listeria monocytogenes in premises and products of small food business operators in Northern Ireland. Food Control, 2018, 87, 70-78. doi:
dc.description.abstractListeriosis is a foodborne disease, with a high mortality rate, that predominantly affects the elderly. Under European Union legislation, EC 2073/2005, food business operators are encouraged to undertake sampling to ensure that the food processing environment, and required to ensure that food products, are free of Listeria monocytogenes. To determine the prevalence of L. monocytogenes in smaller food processing facilities in Northern Ireland, 24 companies submitted six processing environment swabs and two food samples every two months for eighteen months (July 2015 to November 2016) for L. monocytogenes examination. The prevalence of L. monocytogenes was 4.6% in food samples, and 6.3% in processing environment swabs. Over the duration of the study, 96 isolates of L. monocytogenes were obtained, one from each positive sample, except for two meat samples that had >100 cfu/g, where two isolates were obtained from each sample. No seasonality in occurrence of L. monocytogenes was seen for food isolates but significantly higher numbers of positive processing environment swabs were found in the warmer months of May, July and September (p = .007). Pulsed Field Gel Electrophoresis (PFGE) analysis revealed the presence of 27 pulsotypes; 9 pulsotypes were shared between different facilities and 9 were persistent. Based on a Combase predictive growth model, 77.5% (n = 130) of the foods tested were predicted to support the growth of L. monocytogenes. All of the isolates carried the pathogenicity genes inlA and actA and 71.4% carried qacH, which confers resistance to quaternary ammonium compounds which are frequently used in sanitizers. Whole genome sequencing of the isolates allowed multi-locus sequence typing to be undertaken. The data indicated that the sequence types identified included those with disease-causing ability, highlighting the disease-causing potential of the isolates.en_US
dc.relation.ispartofseriesFood Control;Vol. 87
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.subjectListeria monocytogenesen_US
dc.subjectFood safetyen_US
dc.subjectFood processingen_US
dc.titlePrevalence and persistence of Listeria monocytogenes in premises and products of small food business operators in Northern Irelanden_US
dc.contributor.sponsorSafeFood, The Food Safety Promotion Boarden_US

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