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dc.contributor.authorAngeliki, Angelopoulou
dc.contributor.authorHolohan, Ross
dc.contributor.authorRea, Mary C.
dc.contributor.authorWarda, Alicja K.
dc.contributor.authorHill, Colin
dc.contributor.authorRoss, R Paul
dc.date.accessioned2020-06-29T16:05:10Z
dc.date.available2020-06-29T16:05:10Z
dc.date.issued2019-08-07
dc.identifier.citationAngeliki, A., Holohan, R., Rea, M., Warda, A., Hill, C. and Ross, R. Bovine mastitis is a polymicrobial disease requiring a polydiagnostic approach. International Dairy Journal, 2019, 99, 104539. doi: https://doi.org/10.1016/j.idairyj.2019.104539en_US
dc.identifier.urihttp://hdl.handle.net/11019/2082
dc.descriptionpeer-revieweden_US
dc.description.abstractBovine mastitis, an inflammation of the udder, is associated with increases in milk somatic cell count usually resulting from bacterial infection. We analysed 50 mastitic milk samples via cultivation, 16S rRNA sequencing and a combination of the two (culturomics) to define the complete microbial content of the milk. Most samples contained over 10,000 cfu mL-1 total bacterial counts including isolates that were haemolysin positive (n = 36). Among colonies isolated from blood agar plates, Streptococcus uberis was dominant (11/50) followed by Streptococcus dysgalactiae (6/50), Pseudomonas (6/50), Enterococcus faecalis (6/50), Escherichia coli (6/50), Staphylococcus argenteus (4/50), Bacillus (4/50) and Staphylococcus aureus (2/50). 16S rRNA profiling revealed that amplicons were dominated by Rhodococcus, Staphylococcus, Streptococcus and Pseudomonas. A higher inter-sample diversity was noted in the 16S rRNA readouts, which was not always reflected in the plating results. The combination of the two methods highlights the polymicrobial complexity of bovine mastitis.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofseriesInternational Dairy Journal;
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectBovine mastitisen_US
dc.subjectSomatic cell counten_US
dc.subjectMilken_US
dc.subject16S rRNA sequencingen_US
dc.subject16S rRNA profilingen_US
dc.subjectRhodococcusen_US
dc.subjectStaphylococcusen_US
dc.subjectStreptococcusen_US
dc.subjectPseudomonasen_US
dc.titleBovine mastitis is a polymicrobial disease requiring a polydiagnostic approachen_US
dc.typeArticleen_US
dc.embargo.terms2020-08-07en_US
dc.identifier.doihttps://doi.org/10.1016/j.idairyj.2019.104539
dc.contributor.sponsorScience Foundation Irelanden_US
dc.contributor.sponsorGrantNumberSFI/12/RC/2273en_US


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