Show simple item record

dc.contributor.authorVegh, Peter
dc.contributor.authorMagee, David A.
dc.contributor.authorNalpas, Nicolas C.
dc.contributor.authorBryan, Kenneth
dc.contributor.authorMcCabe, Matthew S.
dc.contributor.authorBrowne, John A.
dc.contributor.authorConlon, Kevin M.
dc.contributor.authorGordon, Stephen V.
dc.contributor.authorBradley, Daniel G.
dc.contributor.authorMacHugh, David E.
dc.contributor.authorLynn, David J.
dc.date.accessioned2020-06-30T11:53:27Z
dc.date.available2020-06-30T11:53:27Z
dc.date.issued2014-11-05
dc.identifier.citationVegh, P., Magee, D., Nalpas, N., Bryan, K., McCabe, M., Browne, J., Conlon, K., Gordon, S., Bradley, D., MacHugh, D. and Lynn, D. MicroRNA profiling of the bovine alveolar macrophage response to Mycobacterium bovis infection suggests pathogen survival is enhanced by microRNA regulation of endocytosis and lysosome trafficking. Tuberculosis, 2015, 95(1), 60-67. doi: http://dx.doi.org/10.1016/j.tube.2014.10.011en_US
dc.identifier.urihttp://hdl.handle.net/11019/2102
dc.descriptionpeer-revieweden_US
dc.description.abstractMycobacterium bovis, the causative agent of bovine tuberculosis, a major problem for global agriculture, spreads via an airborne route and is taken up by alveolar macrophages (AM) in the lung. Here, we describe the first next-generation sequencing (RNA-seq) approach to temporally profile miRNA expression in primary bovine AMs post-infection with M. bovis. One, six, and forty miRNAs were identified as significantly differentially expressed at 2, 24 and 48 h post-infection, respectively. The differential expression of three miRNAs (bta-miR-142-5p, bta-miR-146a, and bta-miR-423-3p) was confirmed by RT-qPCR. Pathway analysis of the predicted mRNA targets of differentially expressed miRNAs suggests that these miRNAs preferentially target several pathways that are functionally relevant for mycobacterial pathogenesis, including endocytosis and lysosome trafficking, IL-1 signalling and the TGF-β pathway. Over-expression studies using a bovine macrophage cell-line (Bomac) reveal the targeting of two key genes in the innate immune response to M. bovis, IL-1 receptor-associated kinase 1 (IRAK1) and TGF-β receptor 2 (TGFBR2), by miR-146. Taken together, our study suggests that miRNAs play a key role in tuning the complex interplay between M. bovis survival strategies and the host immune response.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofseriesTuberculosis;Vol. 95 (1)
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectBos taurusen_US
dc.subjectAlveolar macrophageen_US
dc.subjectmicroRNAen_US
dc.subjectRNA-seqen_US
dc.subjectMycobacterium bovisen_US
dc.subjectTuberculosisen_US
dc.titleMicroRNA profiling of the bovine alveolar macrophage response to Mycobacterium bovis infection suggests pathogen survival is enhanced by microRNA regulation of endocytosis and lysosome traffickingen_US
dc.typeArticleen_US
dc.embargo.terms2015-11-05en_US
dc.identifier.rmis6082
dc.identifier.doihttp://dx.doi.org/10.1016/j.tube.2014.10.011
dc.contributor.sponsorTeagascen_US
dc.contributor.sponsorScience Foundation Irelanden_US
dc.contributor.sponsorDepartment of Agriculture, Food and the Marineen_US
dc.contributor.sponsorTeagasc Walsh Fellowship Programmeen_US
dc.contributor.sponsorGrantNumberRMIS 6082en_US
dc.contributor.sponsorGrantNumberSFI/08/IN.1/B2038en_US
dc.contributor.sponsorGrantNumberRSF 06 405en_US
refterms.dateFOA2015-11-05T00:00:00Z


Files in this item

Thumbnail
Name:
Publisher version
Thumbnail
Name:
MicroRNA-profiling-of-the-bovi ...
Size:
968.2Kb
Format:
PDF
Description:
main article

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-ShareAlike 3.0 United States
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 3.0 United States