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dc.contributor.authorQuinn, Erinn M.
dc.contributor.authorSlattery, Helen
dc.contributor.authorThompson, Aoife P.
dc.contributor.authorKilcoyne, Michelle
dc.contributor.authorJoshi, Lokesh
dc.contributor.authorHickey, Rita M.
dc.date.accessioned2020-07-02T14:08:11Z
dc.date.available2020-07-02T14:08:11Z
dc.date.issued2018-12-03
dc.identifier.citationQuinn, E.M.; Slattery, H.; Thompson, A.P.; Kilcoyne, M.; Joshi, L.; Hickey, R.M. Mining Milk for Factors which Increase the Adherence of Bifidobacterium longum subsp. infantis to Intestinal Cells. Foods 2018, 7, 196. doi: https://dx.doi.org/10.3390/foods7120196en_US
dc.identifier.urihttp://hdl.handle.net/11019/2124
dc.descriptionpeer-revieweden_US
dc.description.abstractBifidobacteria play a vital role in human nutrition and health by shaping and maintaining the gut ecosystem. In order to exert a beneficial effect, a sufficient population of bifidobacteria must colonise the host. In this study, we developed a miniaturised high-throughput in vitro assay for assessing the colonising ability of bacterial strains in human cells. We also investigated a variety of components isolated from different milk sources for their ability to increase the adherence of Bifidobacterium longum subsp. infantis ATCC 15697, a common member of the gastrointestinal microbiota of breastfed infants, to HT-29 cells. Both conventional and miniaturised colonisation assays were employed to examine the effect of 13 different milk-derived powders on bacterial adherence, including positive controls which had previously resulted in increased bifidobacterial adherence (human milk oligosaccharides and a combination of 3′- and 6′-sialylactose) to intestinal cells. Immunoglobulin G enriched from bovine whey and goat milk oligosaccharides resulted in increased adhesion (3.3- and 8.3-fold, respectively) of B. infantis to the intestinal cells and the miniaturised and conventional assays were found to yield comparable and reproducible results. This study highlights the potential of certain milk components to favourably modulate adhesion of bifidobacteria to human intestinal cells.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofseriesFoods;Vol. 7 (12)
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectBifidobacteriumen_US
dc.subjectadhesionen_US
dc.subjectmilk oligosaccharidesen_US
dc.subjectmilk powdersen_US
dc.subjectHT-29 cellsen_US
dc.titleMining Milk for Factors which Increase the Adherence of Bifidobacterium longum subsp. infantis to Intestinal Cellsen_US
dc.typeArticleen_US
dc.identifier.doihttps://dx.doi.org/10.3390/foods7120196
dc.contributor.sponsorTeagasc Walsh Fellowship Programmeen_US
refterms.dateFOA2020-07-02T14:08:11Z


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