Show simple item record

dc.contributor.authorLynch, Caoimhe
dc.contributor.authorHawkins, Kayleigh
dc.contributor.authorLynch, Helen
dc.contributor.authorEgan, John
dc.contributor.authorBolton, Declan J.
dc.contributor.authorCoffey, Aidan
dc.contributor.authorLucey, Brigid
dc.date.accessioned2020-07-02T14:45:00Z
dc.date.available2020-07-02T14:45:00Z
dc.date.issued2019-11-09
dc.identifier.citationLynch, C., Hawkins, K., Lynch, H. et al. Investigation of molecular mechanisms underlying tetracycline resistance in thermophilic Campylobacter spp. suggests that previous reports of tet(A)-mediated resistance in these bacteria are premature. Gut Pathog 11, 56 (2019). https://doi.org/10.1186/s13099-019-0338-1en_US
dc.identifier.urihttps://doi.org/10.1186/s13099-019-0338-1
dc.identifier.urihttp://hdl.handle.net/11019/2131
dc.descriptionpeer-revieweden_US
dc.description.abstractThe true prevalence of tet(A), which codes for a tetracycline efflux pump, in thermophilic Camplyobacter spp. requires clarification after reports emerged in Iran (2014) and Kenya (2016) of the novel detection of tet(A) in Campylobacter. During our investigation of antibiotic resistance mechanisms in a sample of Irish thermophilic Campylobacter broiler isolates, it was determined that 100% of tetracycline-resistant isolates (n = 119) harboured tet(O). Accessory tetracycline-resistance mechanisms were considered as tetracycline minimum inhibitory concentrations ranged from 4 to ≥ 64 mg/L. Primers previously reported for the detection of tet(A) in Campylobacter failed to produce an amplicon using a positive control strain (Escherichia coli K12 SK1592 containing the pBR322 plasmid) and a selection of Campylobacter isolates. Accordingly, we designed new tet(A)-targeting primers on SnapGene2.3.2 that successfully generated a 407 bp product from the positive control strain only. Further in silico analysis using BLASTn and SnapGene2.3.2 revealed that previously reported Campylobacter tet(A) sequences deposited on GenBank shared 100% homology with Campylobacter tet(O). We postulate that this gave rise to the erroneous report of a high tet(A) prevalence among a pool of Kenyan broiler Campylobacter isolates that were tested using primers designed based on these apparent tet(A) sequences. In conclusion, further work would be required to determine whether the homology between tet(A) potentially present in Campylobacter and known tet(A) genes would be sufficient to allow amplification using the primers designed in our study. Finally, the existence of tet(A) in thermophilic Campylobacter spp. remains to be demonstrated.en_US
dc.language.isoenen_US
dc.publisherBiomed Centralen_US
dc.relation.ispartofseriesGut Pathogens;
dc.subjectCampylobacteren_US
dc.subjectTetracyclineen_US
dc.subjecttet(A)en_US
dc.subjecttet(O)en_US
dc.subjectAntibiotic resistanceen_US
dc.subjectAntimicrobial susceptibilityen_US
dc.subjectAntibiotic resistance mechanismsen_US
dc.subjectMobile genetic elementsen_US
dc.subjectPlasmidsen_US
dc.titleInvestigation of molecular mechanisms underlying tetracycline resistance in thermophilic Campylobacter spp. suggests that previous reports of tet(A)-mediated resistance in these bacteria are prematureen_US
dc.typeArticleen_US
dc.date.updated2019-11-10T05:18:28Z
dc.language.rfc3066en
dc.rights.holderThe Author(s)
dc.contributor.sponsorDepartment of Agriculture, Food and the Marineen_US
dc.contributor.sponsorTeagasc Walsh Fellowship Programmeen_US
dc.contributor.sponsorGrantNumber15/F/641en_US
dc.contributor.sponsorGrantNumber2017265en_US
refterms.dateFOA2020-07-02T14:45:01Z


Files in this item

Thumbnail
Name:
13099_2019_Article_338.pdf
Size:
743.7Kb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record