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dc.contributor.authorPereira-Fantini, Prue
dc.contributor.authorByars, Sean G
dc.contributor.authorPitt, James
dc.contributor.authorLapthorne, Susan
dc.contributor.authorFouhy, Fiona
dc.contributor.authorCotter, Paul D.
dc.contributor.authorBines, Julie E.
dc.date.accessioned2020-07-03T11:30:47Z
dc.date.available2020-07-03T11:30:47Z
dc.date.issued2017-02-23
dc.identifier.citationPereira-Fantini, P., Byars, S., Pitt, J. et al. Unravelling the metabolic impact of SBS-associated microbial dysbiosis: Insights from the piglet short bowel syndrome model. Sci Rep 7, 43326 (2017). https://doi.org/10.1038/srep43326en_US
dc.identifier.urihttp://hdl.handle.net/11019/2145
dc.descriptionpeer-revieweden_US
dc.description.abstractLiver disease is a major source of morbidity and mortality in children with short bowel syndrome (SBS). SBS-associated microbial dysbiosis has recently been implicated in the development of SBS-associated liver disease (SBS-ALD), however the pathological implications of this association have not been explored. In this study high-throughput sequencing of colonic content from the well-validated piglet SBS-ALD model was examined to determine alterations in microbial communities, and concurrent metabolic alterations identified in urine samples via targeted mass spectrometry approaches (GC-MS, LC-MS, FIA-MS) further uncovered impacts of microbial disturbance on metabolic outcomes in SBS-ALD. Multi-variate analyses were performed to elucidate contributing SBS-ALD microbe and metabolite panels and to identify microbe-metabolite interactions. A unique SBS-ALD microbe panel was clearest at the genus level, with discriminating bacteria predominantly from the Firmicutes and Bacteroidetes phyla. The SBS-ALD metabolome included important alterations in the microbial metabolism of amino acids and the mitochondrial metabolism of branched chain amino acids. Correlation analysis defined microbe-metabolite clustering patterns unique to SBS-ALD and identified a metabolite panel that correlates with dysbiosis of the gut microbiome in SBS.en_US
dc.language.isoenen_US
dc.publisherSpringer Natureen_US
dc.relation.ispartofseriesScientific Reports;Vol. 7 (1)
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectLiver diseaseen_US
dc.subjectmicrobial dysbiosisen_US
dc.subjectshort bowel syndromeen_US
dc.subjectSBS-ALDen_US
dc.subjectPaediatric researchen_US
dc.titleUnravelling the metabolic impact of SBS-associated microbial dysbiosis: Insights from the piglet short bowel syndrome modelen_US
dc.typeArticleen_US
dc.identifier.doihttps://dx.doi.org/10.1038/srep43326
dc.contributor.sponsorScience Foundation Irelanden_US
dc.contributor.sponsorGrantNumberSFI/12/RC/2273en_US
refterms.dateFOA2020-07-03T11:30:47Z


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