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dc.contributor.authorBowen, Jenna M.
dc.contributor.authorCormican, Paul
dc.contributor.authorLister, Susan J.
dc.contributor.authorMcCabe, Matthew S.
dc.contributor.authorDuthie, Carol-Anne
dc.contributor.authorRoehe, Rainer
dc.contributor.authorDewhurst, Richard J.
dc.date.accessioned2020-07-28T10:52:07Z
dc.date.available2020-07-28T10:52:07Z
dc.date.issued2020-04-24
dc.identifier.citationBowen JM, Cormican P, Lister SJ, McCabe MS, Duthie C-A, Roehe R, et al. (2020) Links between the rumen microbiota, methane emissions and feed efficiency of finishing steers offered dietary lipid and nitrate supplementation. PLoS ONE 15(4): e0231759. https://doi.org/10.1371/journal.pone.0231759en_US
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11019/2189
dc.descriptionpeer-revieweden_US
dc.description.abstractRuminant methane production is a significant energy loss to the animal and major contributor to global greenhouse gas emissions. However, it also seems necessary for effective rumen function, so studies of anti-methanogenic treatments must also consider implications for feed efficiency. Between-animal variation in feed efficiency represents an alternative approach to reducing overall methane emissions intensity. Here we assess the effects of dietary additives designed to reduce methane emissions on the rumen microbiota, and explore relationships with feed efficiency within dietary treatment groups. Seventy-nine finishing steers were offered one of four diets (a forage/concentrate mixture supplemented with nitrate (NIT), lipid (MDDG) or a combination (COMB) compared to the control (CTL)). Rumen fluid samples were collected at the end of a 56 d feed efficiency measurement period. DNA was extracted, multiplexed 16s rRNA libraries sequenced (Illumina MiSeq) and taxonomic profiles were generated. The effect of dietary treatments and feed efficiency (within treatment groups) was conducted both overall (using non-metric multidimensional scaling (NMDS) and diversity indexes) and for individual taxa. Diet affected overall microbial populations but no overall difference in beta-diversity was observed. The relative abundance of Methanobacteriales (Methanobrevibacter and Methanosphaera) increased in MDDG relative to CTL, whilst VadinCA11 (Methanomassiliicoccales) was decreased. Trimethylamine precursors from rapeseed meal (only present in CTL) probably explain the differences in relative abundance of Methanomassiliicoccales. There were no differences in Shannon indexes between nominal low or high feed efficiency groups (expressed as feed conversion ratio or residual feed intake) within treatment groups. Relationships between the relative abundance of individual taxa and feed efficiency measures were observed, but were not consistent across dietary treatments.en_US
dc.language.isoenen_US
dc.publisherPublic Library of Science (PLoS)en_US
dc.relation.ispartofseriesPLOS One;15
dc.rightsAttribution-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-sa/3.0/us/*
dc.subjectrumen microbiotaen_US
dc.subjectmethane emissionsen_US
dc.subjectfeed efficiencyen_US
dc.subjectsteersen_US
dc.subjectdietary lipiden_US
dc.subjectnitrate supplementationen_US
dc.titleLinks between the rumen microbiota, methane emissions and feed efficiency of finishing steers offered dietary lipid and nitrate supplementationen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0231759
dc.contributor.sponsorTeagasc Walsh Fellowship Programmeen_US
dc.contributor.sponsorAHDB Beef and Lamben_US
dc.contributor.sponsorthe Scottish Governmenten_US
dc.contributor.sponsorDEFRAen_US
dc.contributor.sponsorGrantNumber2012026en_US
dc.contributor.sponsorGrantNumber66714en_US
dc.source.volume15
dc.source.issue4
dc.source.beginpagee0231759
refterms.dateFOA2020-07-28T10:52:07Z


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Except where otherwise noted, this item's license is described as Attribution-ShareAlike 3.0 United States