Tracking the Dairy Microbiota from Farm Bulk Tank to Skimmed Milk Powder.
Keyword16S rRNA gene amplicon sequencing
bulk tank milk
skimmed milk powder
skimmed milk silo
MetadataShow full item record
StatisticsDisplay Item Statistics
CitationMcHugh AJ, Feehily C, Fenelon MA, Gleeson D, Hill C, Cotter PD. 2020. Tracking the dairy microbiota from farm bulk tank to skimmed milk powder. mSystems 5:e00226-20. https://doi.org/10.1128/mSystems.00226-20
AbstractMicroorganisms from the environment can enter the dairy supply chain at multiple stages, including production, milk collection, and processing, with potential implications for quality and safety. The ability to track these microorganisms can be greatly enhanced by the use of high-throughput DNA sequencing (HTS). Here HTS, both 16S rRNA gene amplicon and shotgun metagenomic sequencing were applied to investigate the microbiomes of fresh mid- and late-lactation milk collected from farm bulk tanks, collection tankers, milk silos, skimmed milk silos, a cream silo, and powder samples to investigate the microbial changes throughout a skim milk powder manufacturing process. 16S rRNA gene analysis established that the microbiota of raw milks from farm bulk tanks and in collection tankers were very diverse but that psychrotrophic genera associated with spoilage, Pseudomonas and Acinetobacter, were present in all samples. Upon storage within the whole-milk silo at the processing facility, the species Pseudomonas fluorescens and Acinetobacter baumannii became dominant. The skimmed milk powder generated during the mid-lactation period had a microbial composition that was very different from that of raw milk; specifically, two thermophilic genera, Thermus and Geobacillus, were enriched. In contrast, the microbiota of skimmed milk powder generated from late-lactation milk more closely resembled that of the raw milk and was dominated by spoilage-associated psychrotrophic bacteria. This study demonstrates that the dairy microbiota can differ significantly across different sampling days. More specifically, HTS can be used to trace microbial species from raw milks through processing to final powdered products.IMPORTANCE Microorganisms can enter and persist in dairy at several stages of the processing chain. Detection of microorganisms within dairy food processing is currently a time-consuming and often inaccurate process. This study provides evidence that high-throughput sequencing can be used as an effective tool to accurately identify microorganisms along the processing chain. In addition, it demonstrates that the populations of microbes change from raw milk to the end product. Routine implementation of high-throughput sequencing would elucidate the factors that influence population dynamics. This will enable a manufacturer to adopt control measures specific to each stage of processing and respond in an effective manner, which would ultimately lead to increased food safety and quality.
FunderDepartment of Agriculture, Food and the Marine; Science Foundation Ireland; European Union
Grant Number14/F/883; SFI/12/RC/2273; SFI/16/RC/3835; 818368
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 3.0 United States
- The Core and Seasonal Microbiota of Raw Bovine Milk in Tanker Trucks and the Impact of Transfer to a Milk Processing Facility.
- Authors: Kable ME, Srisengfa Y, Laird M, Zaragoza J, McLeod J, Heidenreich J, Marco ML
- Issue date: 2016 Aug 23
- Microbiological quality of milk from farms to milk powder manufacture: an industrial case study.
- Authors: Paludetti LF, Kelly AL, O'Brien B, Jordan K, Gleeson D
- Issue date: 2019 May
- Viable and Total Bacterial Populations Undergo Equipment- and Time-Dependent Shifts during Milk Processing.
- Authors: Kable ME, Srisengfa Y, Xue Z, Coates LC, Marco ML
- Issue date: 2019 Jul 1
- Impacts of Seasonal Housing and Teat Preparation on Raw Milk Microbiota: a High-Throughput Sequencing Study.
- Authors: Doyle CJ, Gleeson D, O'Toole PW, Cotter PD
- Issue date: 2017 Jan 15
- Bacterial spore levels in bulk tank raw milk are influenced by environmental and cow hygiene factors.
- Authors: Martin NH, Kent DJ, Evanowski RL, Zuber Hrobuchak TJ, Wiedmann M
- Issue date: 2019 Nov