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dc.contributor.authorKnorst, Verena
dc.contributor.authorByrne, Stephen
dc.contributor.authorYates, Steven
dc.contributor.authorAsp, Torben
dc.contributor.authorWidmer, Franco
dc.contributor.authorStuder, Bruno
dc.contributor.authorKölliker, Roland
dc.date.accessioned2020-07-31T10:25:41Z
dc.date.available2020-07-31T10:25:41Z
dc.date.issued2018-11-30
dc.identifier.citationKnorst, V., Byrne, S., Yates, S. et al. Pooled DNA sequencing to identify SNPs associated with a major QTL for bacterial wilt resistance in Italian ryegrass (Lolium multiflorum Lam.). Theor Appl Genet 132, 947–958 (2019). https://doi.org/10.1007/s00122-018-3250-zen_US
dc.identifier.issn0040-5752
dc.identifier.issn1432-2242
dc.identifier.urihttp://hdl.handle.net/11019/2206
dc.descriptionpeer-revieweden_US
dc.description.abstractItalian ryegrass (Lolium multiflorum Lam.) is one of the most important forage grass species in temperate regions. Its yield, quality and persistency can significantly be reduced by bacterial wilt, a serious disease caused by Xanthomonas translucens pv. graminis. Although a major QTL for bacterial wilt resistance has previously been reported, detailed knowledge on underlying genes and DNA markers to allow for efficient resistance breeding strategies is currently not available. We used pooled DNA sequencing to characterize a major QTL for bacterial wilt resistance of Italian ryegrass and to develop inexpensive sequence-based markers to efficiently target resistance alleles for marker-assisted recurrent selection. From the mapping population segregating for the QTL, DNA of 44 of the most resistant and 44 of the most susceptible F1 individuals was pooled and sequenced using the Illumina HiSeq 2000 platform. Allele frequencies of 18 × 106 single nucleotide polymorphisms (SNP) were determined in the resistant and susceptible pool. A total of 271 SNPs on 140 scaffold sequences of the reference parental genome showed significantly different allele frequencies in both pools. We converted 44 selected SNPs to KASP™ markers, genetically mapped these proximal to the major QTL and thus validated their association with bacterial wilt resistance. This study highlights the power of pooled DNA sequencing to efficiently target binary traits in biparental mapping populations. It delivers genome sequence data, SNP markers and potential candidate genes which will allow to implement marker-assisted strategies to fix bacterial wilt resistance in outcrossing breeding populations of Italian ryegrass.en_US
dc.language.isoenen_US
dc.publisherSpringer Science and Business Media LLCen_US
dc.relation.ispartofseriesTheoretical and Applied Genetics;132
dc.rightsAttribution-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-sa/3.0/us/*
dc.subjectdna sequencingen_US
dc.subjectSNPen_US
dc.subjectQTLen_US
dc.subjectbacterial wilt resistanceen_US
dc.subjectItalian ryegrassen_US
dc.titlePooled DNA sequencing to identify SNPs associated with a major QTL for bacterial wilt resistance in Italian ryegrass (Lolium multiflorum Lam.)en_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1007/s00122-018-3250-z
dc.contributor.sponsorSwiss National Science Foundationen_US
dc.contributor.sponsorGrantNumber31003A_138358en_US
dc.source.volume132
dc.source.issue4
dc.source.beginpage947-958
refterms.dateFOA2020-07-31T10:25:41Z


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