Transcriptome sequencing of Festulolium accessions under salt stress
Citation
Teshome, A., Byrne, S.L., Didion, T. et al. Transcriptome sequencing of Festulolium accessions under salt stress. BMC Res Notes 12, 311 (2019). https://doi.org/10.1186/s13104-019-4349-2Abstract
Objectives The objective of this study was to establish transcriptome assemblies of Festulolium hybrids under salt stress, and identify genes regulated across the hybrids in response to salt stress. The development of transcriptome assemblies for Festulolium hybrids and cataloguing of genes regulated under salt stress will facilitate further downstream studies. Results Plants were grown at three salt concentrations (0.5%, 1% and 1.5%) and phenotypic and transcriptomic data was collected. Salt stress was confirmed by progressive loss of green leaves as salt concentration increased from 0 to 1.5%. We generated de-novo transcriptome assemblies for two Festulolium pabulare festucoid genotypes, for a single Festulolium braunii genotype, and a single F. pabulare loloid genotype. We also identified 1555 transcripts that were up regulated and 1264 transcripts that were down regulated in response to salt stress in the Festulolium hybrids. Some of the identified transcripts showed significant sequence similarity with genes known to be regulated during salt and other abiotic stresses.Funder
European Union; Marie Sklodowska-Curie ActionsGrant Number
FP7-KBBE-2011-5-289461; CLNE/2017/364ae974a485f413a2113503eed53cd6c53
https://doi.org/10.1186/s13104-019-4349-2