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dc.contributor.authorLynch, Caoimhe T.
dc.contributor.authorLynch, Helen
dc.contributor.authorBurke, Sarah
dc.contributor.authorHawkins, Kayleigh
dc.contributor.authorButtimer, Colin
dc.contributor.authorMcCarthy, Conor
dc.contributor.authorEgan, John
dc.contributor.authorWhyte, Paul
dc.contributor.authorBolton, Declan
dc.contributor.authorCoffey, Aidan
dc.contributor.authorLucey, Brigid
dc.date.accessioned2020-08-04T14:43:53Z
dc.date.available2020-08-04T14:43:53Z
dc.date.issued2020-06-08
dc.identifier.citationLynch, C.T.; Lynch, H.; Burke, S.; Hawkins, K.; Buttimer, C.; Mc Carthy, C.; Egan, J.; Whyte, P.; Bolton, D.; Coffey, A.; Lucey, B. Antimicrobial Resistance Determinants Circulating among Thermophilic Campylobacter Isolates Recovered from Broilers in Ireland Over a One-Year Period. Antibiotics 2020, 9, 308. https://doi.org/10.3390/antibiotics9060308en_US
dc.identifier.issn2079-6382
dc.identifier.urihttp://hdl.handle.net/11019/2255
dc.descriptionpeer-revieweden_US
dc.description.abstractCampylobacteriosis is the leading cause of human bacterial gastroenteritis, very often associated with poultry consumption. Thermophilic Campylobacter (Campylobacter jejuni and Campylobacter coli) isolates (n = 158) recovered from broiler neck skin and caecal contents in Ireland over a one-year period, resistant to at least one of three clinically relevant antimicrobial classes, were screened for resistance determinants. All ciprofloxacin-resistant isolates (n = 99) harboured the C257T nucleotide mutation (conferring the Thr-86-Ile substitution) in conjunction with other synonymous and nonsynonymous mutations, which may have epidemiological value. The A2075G nucleotide mutation and amino acid substitutions in L4 and L22 were detected in all erythromycin-resistant isolates (n = 5). The tetO gene was detected in 100% (n = 119) of tetracycline-resistant isolates and three of which were found to harbour the mosaic tetracycline resistance gene tetO/32/O. Two streptomycin-resistant C. jejuni isolates (isolated from the same flock) harboured ant(6)-Ib, located in a multidrug resistance genomic island, containing aminoglycoside, streptothricin (satA) and tetracycline resistance genes (truncated tetO and mosaic tetO/32/O). The ant(6)-Ie gene was identified in two streptomycin-resistant C. coli isolates. This study highlights the widespread acquisition of antimicrobial resistance determinants among chicken-associated Campylobacter isolates, through horizontal gene transfer or clonal expansion of resistant lineages. The stability of such resistance determinants is compounded by the fluidity of mobile genetic element.en_US
dc.language.isoenen_US
dc.publisherMDPI AGen_US
dc.relation.ispartofseriesAntibiotics;9
dc.rightsAttribution-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-sa/3.0/us/*
dc.subjectcampylobacteren_US
dc.subjectantimicrobial resistanceen_US
dc.subjectciprofloxacinen_US
dc.subjecttetracyclineen_US
dc.subjectaminoglycosidesen_US
dc.subjectmacrolidesen_US
dc.titleAntimicrobial Resistance Determinants Circulating among Thermophilic Campylobacter Isolates Recovered from Broilers in Ireland Over a One-Year Perioden_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.3390/antibiotics9060308
dc.contributor.sponsorDepartment of Agriculture Food and the Marineen_US
dc.contributor.sponsorTeagasc Walsh Fellowship Programmeen_US
dc.contributor.sponsorGrantNumber15F641en_US
dc.contributor.sponsorGrantNumber2017265en_US
dc.source.volume9
dc.source.issue6
dc.source.beginpage308
refterms.dateFOA2020-08-04T14:43:53Z


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