Tracking the Dairy Microbiota from Farm Bulk Tank to Skimmed Milk Powder
Keyword
dairyprocessing
metagenomics
skimmed milk powder
16S rRNA gene amplicon sequencing
bulk tank milk
whole-milk silo
skimmed milk silo
collection tanker
microbiota
Date
2020-04-07
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McHugh AJ, Feehily C, Fenelon MA, Gleeson D, Hill C, Cotter PD. 2020. Tracking the dairy microbiota from farm bulk tank to skimmed milk powder. mSystems 5:e00226-20. https://doi.org/10.1128/mSystems.00226-20Abstract
Microorganisms from the environment can enter the dairy supply chain at multiple stages, including production, milk collection, and processing, with potential implications for quality and safety. The ability to track these microorganisms can be greatly enhanced by the use of high-throughput DNA sequencing (HTS). Here HTS, both 16S rRNA gene amplicon and shotgun metagenomic sequencing were applied to investigate the microbiomes of fresh mid- and late-lactation milk collected from farm bulk tanks, collection tankers, milk silos, skimmed milk silos, a cream silo, and powder samples to investigate the microbial changes throughout a skim milk powder manufacturing process. 16S rRNA gene analysis established that the microbiota of raw milks from farm bulk tanks and in collection tankers were very diverse but that psychrotrophic genera associated with spoilage, Pseudomonas and Acinetobacter, were present in all samples. Upon storage within the whole-milk silo at the processing facility, the species Pseudomonas fluorescens and Acinetobacter baumannii became dominant. The skimmed milk powder generated during the mid-lactation period had a microbial composition that was very different from that of raw milk; specifically, two thermophilic genera, Thermus and Geobacillus, were enriched. In contrast, the microbiota of skimmed milk powder generated from late-lactation milk more closely resembled that of the raw milk and was dominated by spoilage-associated psychrotrophic bacteria. This study demonstrates that the dairy microbiota can differ significantly across different sampling days. More specifically, HTS can be used to trace microbial species from raw milks through processing to final powdered products.Funder
Department of Agriculture, Food and the Marine; Science Foundation Ireland; European UnionGrant Number
14/F/883; SFI/12/RC/2273; SFI/16/RC/3835; 818368ae974a485f413a2113503eed53cd6c53
https://doi.org/10.1128/mSystems.00226-20
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