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dc.contributor.authorMcHugh, Aoife
dc.contributor.authorFeehily, Conor
dc.contributor.authorFenelon, Mark
dc.contributor.authorGleeson, David E
dc.contributor.authorHill, Colin
dc.contributor.authorCotter, Paul D.
dc.date.accessioned2020-08-04T14:44:11Z
dc.date.available2020-08-04T14:44:11Z
dc.date.issued2020-04-07
dc.identifier.citationMcHugh AJ, Feehily C, Fenelon MA, Gleeson D, Hill C, Cotter PD. 2020. Tracking the dairy microbiota from farm bulk tank to skimmed milk powder. mSystems 5:e00226-20. https://doi.org/10.1128/mSystems.00226-20en_US
dc.identifier.issn2379-5077
dc.identifier.urihttp://hdl.handle.net/11019/2256
dc.descriptionpeer-revieweden_US
dc.description.abstractMicroorganisms from the environment can enter the dairy supply chain at multiple stages, including production, milk collection, and processing, with potential implications for quality and safety. The ability to track these microorganisms can be greatly enhanced by the use of high-throughput DNA sequencing (HTS). Here HTS, both 16S rRNA gene amplicon and shotgun metagenomic sequencing were applied to investigate the microbiomes of fresh mid- and late-lactation milk collected from farm bulk tanks, collection tankers, milk silos, skimmed milk silos, a cream silo, and powder samples to investigate the microbial changes throughout a skim milk powder manufacturing process. 16S rRNA gene analysis established that the microbiota of raw milks from farm bulk tanks and in collection tankers were very diverse but that psychrotrophic genera associated with spoilage, Pseudomonas and Acinetobacter, were present in all samples. Upon storage within the whole-milk silo at the processing facility, the species Pseudomonas fluorescens and Acinetobacter baumannii became dominant. The skimmed milk powder generated during the mid-lactation period had a microbial composition that was very different from that of raw milk; specifically, two thermophilic genera, Thermus and Geobacillus, were enriched. In contrast, the microbiota of skimmed milk powder generated from late-lactation milk more closely resembled that of the raw milk and was dominated by spoilage-associated psychrotrophic bacteria. This study demonstrates that the dairy microbiota can differ significantly across different sampling days. More specifically, HTS can be used to trace microbial species from raw milks through processing to final powdered products.en_US
dc.language.isoenen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.ispartofseriesmSystems;Vol 5
dc.rightsAttribution-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-sa/3.0/us/*
dc.subjectdairyen_US
dc.subjectprocessingen_US
dc.subjectmetagenomicsen_US
dc.subjectskimmed milk powderen_US
dc.subject16S rRNA gene amplicon sequencingen_US
dc.subjectbulk tank milken_US
dc.subjectwhole-milk siloen_US
dc.subjectskimmed milk siloen_US
dc.subjectcollection tankeren_US
dc.subjectmicrobiotaen_US
dc.titleTracking the Dairy Microbiota from Farm Bulk Tank to Skimmed Milk Powderen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1128/mSystems.00226-20
dc.contributor.sponsorDepartment of Agriculture, Food and the Marineen_US
dc.contributor.sponsorScience Foundation Irelanden_US
dc.contributor.sponsorEuropean Unionen_US
dc.contributor.sponsorGrantNumber14/F/883en_US
dc.contributor.sponsorGrantNumberSFI/12/RC/2273en_US
dc.contributor.sponsorGrantNumberSFI/16/RC/3835en_US
dc.contributor.sponsorGrantNumber818368en_US
dc.source.volume5
dc.source.issue2
refterms.dateFOA2020-08-04T14:44:11Z


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