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dc.contributor.authorBirsan, Rares I.
dc.contributor.authorWilde, Peter
dc.contributor.authorWaldron, Keith W.
dc.contributor.authorRai, Dilip K.
dc.date.accessioned2020-08-04T15:14:46Z
dc.date.available2020-08-04T15:14:46Z
dc.date.issued2019-09-07
dc.identifier.citationBirsan, R.I.; Wilde, P.; Waldron, K.W.; Rai, D.K. Recovery of Polyphenols from Brewer’s Spent Grains. Antioxidants 2019, 8, 380. https://doi.org/10.3390/antiox8090380en_US
dc.identifier.issn2076-3921
dc.identifier.urihttp://hdl.handle.net/11019/2257
dc.descriptionpeer-revieweden_US
dc.description.abstractThe recovery of antioxidant polyphenols from light, dark and mix brewer’s spent grain (BSG) using conventional maceration, microwave and ultrasound assisted extraction was investigated. Total polyphenols were measured in the crude (60% acetone), liquor extracts (saponified with 0.75% NaOH) and in their acidified ethyl acetate (EtOAc) partitioned fractions both by spectrophotometry involving Folin–Ciocalteu reagent and liquid-chromatography-tandem mass spectrometry (LC-MS/MS) methods. Irrespective of the extraction methods used, saponification of BSG yielded higher polyphenols than in the crude extracts. The EtOAc fractionations yielded the highest total phenolic content (TPC) ranging from 3.01 ± 0.19 to 4.71 ± 0.28 mg gallic acid equivalent per g of BSG dry weight. The corresponding total polyphenols quantified by LC-MS/MS ranged from 549.9 ± 41.5 to 2741.1 ± 5.2 µg/g of BSG dry weight. Microwave and ultrasound with the parameters and equipment used did not improve the total polyphenol yield when compared to the conventional maceration method. Furthermore, the spectrophotometric quantification of the liquors overestimated the TPC, while the LC-MS/MS quantification gave a closer representation of the total polyphenols in all the extracts. The total polyphenols were in the following order in the EtOAc fractions: BSG light > BSG Mix > BSG dark, and thus suggested BSG light as a sustainable, low cost source of natural antioxidants that may be tapped for applications in food and phytopharmaceutical industriesen_US
dc.language.isoenen_US
dc.publisherMDPI AGen_US
dc.relation.ispartofseriesAntioxidants;8
dc.rightsAttribution-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-sa/3.0/us/*
dc.subjectbewer's spent grainen_US
dc.subjectpolyphenolsen_US
dc.subjectmicrowave assisted extractionen_US
dc.subjectultrasound assisted extractionen_US
dc.subjectliquid chromatography-mass spectrometryen_US
dc.titleRecovery of Polyphenols from Brewer’s Spent Grainsen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.3390/antiox8090380
dc.contributor.sponsorTeagasc Walsh Fellowship Programmeen_US
dc.contributor.sponsorGrantNumber2014027en_US
dc.source.volume8
dc.source.issue9
dc.source.beginpage380
refterms.dateFOA2020-08-04T15:14:47Z


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