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dc.contributor.authorMooney, D
dc.contributor.authorCoxon, C
dc.contributor.authorRichards, Karl G.
dc.contributor.authorGill, L.W.
dc.contributor.authorMellander, Per-Erik
dc.contributor.authorDanaher, Martin
dc.creatorD., Mooney
dc.date.accessioned2020-08-04T15:20:46Z
dc.date.available2020-08-04T15:20:46Z
dc.date.issued2020-01-10
dc.identifier.citationMooney D, Coxon C, Richards KG, Gill L, Mellander PE, Danaher M. A new sensitive method for the simultaneous chromatographic separation and tandem mass spectrometry detection of anticoccidials, including highly polar compounds, in environmental waters. Journal of Chromatography A 2020;1618:460857; doi https://doi.org/10.1016/j.chroma.2020.460857.en_US
dc.identifier.issn00219673
dc.identifier.urihttp://hdl.handle.net/11019/2258
dc.descriptionpeer-revieweden_US
dc.description.abstractA sensitive and selective method was developed and validated for the determination of 26 anticoccidial compounds (six ionophores and twenty chemical coccidiostats) in surface and groundwater samples at parts-per-quadrillion (pg L−1) to parts-per-trillion (ng L−1) levels by ultra-high performance liquid chromatography with tandem mass spectrometry detection (UHPLC–MS/MS). A range of different analytical columns and mobile phase compositions were evaluated to enhance selectivity and retention of a number of highly polar and basic anticoccidials along with other non-polar coccidiostats. A combined separation, including these problematic polar compounds, was achieved on a phenyl-hexyl column, by binary gradient elution with water/acetonitrile using ammonium formate and formic acid as additives. The anticoccidial residues were extracted from raw, unfiltered, water samples (250 mL) using polymeric divinylbenzene solid phase extraction (SPE) cartridges, with subsequent elution (methanol:acetonitrile:ethyl acetate, 40:40:20, v/v) and concentration prior to determination. The method recovery (at a concentration representative of realistic expected environmental water concentrations based on literature review) ranged from 81% to 105%. The method was successfully validated for 26 anticoccidials, at four concentration levels, in accordance to Commission Decision 2002/657/EC and SANTE/11813/2017 guidelines. Trueness and precision, under within-laboratory reproducibility conditions, ranged from 88% to 111% and 0.9% to 10.3% respectively.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal of Chromatography A
dc.relation.ispartofseriesJournal of Chromatography A;1618
dc.rights© 2020 The Authors. Published by Elsevier B.V.
dc.rightsAttribution-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-sa/3.0/us/*
dc.subjectChemical coccidiostatsen_US
dc.subjectIonophoresen_US
dc.subjectEnvironmental wateren_US
dc.subjectSPEen_US
dc.subjectUHPLC–MS/MSen_US
dc.titleA new sensitive method for the simultaneous chromatographic separation and tandem mass spectrometry detection of anticoccidials, including highly polar compounds, in environmental watersen_US
dc.typeArticleen_US
dc.typeOtheren_US
dc.embargo.terms2021-01-10en_US
dc.identifier.doihttps://doi.org/10.1016/j.chroma.2020.460857
dc.identifier.eid1-s2.0-S0021967320300091
dc.identifier.piiS0021-9673(20)30009-1
dc.relation.volume1618
dc.contributor.sponsorScience Foundation Irelanden_US
dc.contributor.sponsorEuropean Unionen_US
dc.contributor.sponsorTeagasc Walsh Fellowship Programmeen_US
dc.contributor.sponsorIrish Centre for Research in Applied Geosciences (iCRAG)en_US
dc.contributor.sponsorGrantNumber13/RC/2092en_US
dc.contributor.sponsorGrantNumber16/RI/3710en_US
dc.source.volume1618
dc.source.beginpage460857
refterms.dateFOA2020-01-10T00:00:00Z


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© 2020 The Authors. Published by Elsevier B.V.
Except where otherwise noted, this item's license is described as © 2020 The Authors. Published by Elsevier B.V.